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" Gene knockout protocols "
edited by Martin J. Tymms and Ismail Kola.
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BL
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| Record Number
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599935
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| Doc. No
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b429154
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| Title & Author
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Gene knockout protocols\ edited by Martin J. Tymms and Ismail Kola.
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| Publication Statement
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Totowa, NJ :: Humana Press,, c2001.
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| Series Statement
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Methods in molecular biology ;; v. 158
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| Page. NO
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xi, 431 p. :: ill. ;; 24 cm.
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| ISBN
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0896035727 (alk. paper)
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: 9780896035720 (alk. paper)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Overview: gene knockouts -- Isolation and maintenance of primate embryonic stem cells -- Gene targeting in ES cells -- Manipulating mouse embryonic stem cells -- Gene targeting in a centralized facility -- The loxP/CRE system and genome modification -- Creation and use of a Cre recombinase transgenic database -- Choice of mouse strains for gene targeting -- Isolation, microinjection, and transfer of mouse blastocysts -- Aggregatin chimeras: combining ES cells, diploid and tetraploid embryos -- How to study pathologic phenotypes of knockout mice -- Analysis of hematopoietic phenotypes in knockout mouse models -- Isolation of embryonic fibroblasts and their use in the in vitro characterization of gene function -- Influence of genetic background on knockout mouse phenotypes -- Lineage allocation during early embryogenesis: mapping of the neural primordia and application to the analysis of mouse mutants -- Generation of double-knockout, embryonic stem cells -- In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes -- Embryonic stem cells in teh study of hematopoiesis -- Interferon-inducible ES cell expression systems --Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach -- In vivo libraries of large insert transgenic mice for genetic mapping -- Epigenetic effects on transgene expression -- Positional-candidate cloning of genes from mouse mutants -- Genetically engineered mice: husbandry and resources -- Embryo cryopreservation for transgenic mouse lines.
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| Subject
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Gene targeting, Laboratory manuals.
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Transgenic mice, Laboratory manuals.
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| Added Entry
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Tymms, Martin J.
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Kola, Ismail.
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