| Document Type
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BL
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| Record Number
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599936
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| Doc. No
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b429155
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| Title & Author
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Transcription factor protocols\ edited by Martin J. Tymms.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, c2000.
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| Series Statement
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Methods in molecular biology ;; v. 130
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| Page. NO
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x, 306 p. :: ill. ;; 23 cm.
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| ISBN
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0896035735 (acid-free paper)
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: 9780896035737 (acid-free paper)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.
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| Subject
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Transcription factors, Laboratory manuals.
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| Added Entry
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Tymms, Martin J.
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