| Document Type
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BL
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| Record Number
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599943
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| Doc. No
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b429162
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| Title & Author
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Angiotensin protocols /\ edited by Donna H. Wang
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| Series Statement
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Methods in molecular medicine ;; 51
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| Page. NO
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xiv, 530 pages :: illustrations ;; 24 cm
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| ISBN
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0896037630
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: 9780896037632
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| Bibliographies/Indexes
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Includes bibliographical references and index
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| Contents
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pt. 1. Introduction to the renin-angiotensin system -- Historical perspective to the renin-angiotensin system / J.E. Hall -- Three angiotensin paradigms in one patient: etiology of hypertension, glomerular hemodynamics, and long-term glomerular protection / C.L. Laffer -- pt. 2. Transgenic and gene targeting methods -- Production and use of chimeric mice / J. Takaya -- Genetic manipulation of the renin-angiotensin system using cre-loxP-recombinase / Curt D. Sigmund -- Systemic delivery of a transgene in intact animals by a retroviral vector / M.J. Katovich -- Antisense inhibition of the renin-angiotensin system / D. Mohuczy -- pt. 3. Analysis of transcriptional and translational mechanisms -- Analysis of transcriptional control mechanisms I: techniques for characterization of cis-regulatory elements / C.T. Sherman -- Analysis of transcriptional control mechanisms II: techniques for characterization of trans-acting factors / A.R. Brasier -- Analysis of cytosolic proteins that bind to the 5' leader sequence of the angiotensin AT1 receptor by RNA electromobility shift assay / Z. Wu -- In vitro translation of the angiotensin AT1 receptors in wheat-germ extracts / H. Ji -- pt. 4. Assays for determination of mRNA levels -- Quantitation of angiotensin II receptors by competitive reverse-transcription polymerase chain reaction / W. Wu -- Analysis of RNA by Northern-blot hybridization / P.E. Gallagher -- Use of the ribonuclease protection assay for the analysis and characterization of target mRNAs / S.C. Supowit -- In situ hybridization / S. Riggs Runge -- pt. 5. Assays for determination of protein contents and enzyme activity -- Immunohistochemical detection of angiotensin II receptor / Z.-Q. Wang -- Western blotting / N.R. Madamanchi -- Quantification of angiotensin-converting enzyme (ACE) activity / Q.C. Meng -- Quantification of angiotensin peptides / Q.C. Meng -- Radiolabeling of angiotensin peptides / R.C. Speth -- Assays for radiolabel-photoaffinity labeling of angiotensin receptors / J. Perodin -- Radioligand binding assay / G. Thibault -- Autoradiographic localization and quantification of components of the renin-angiotensin system in tissues / S.Y. Chai -- pt. 6. Biochemical studies of the renin-angiotensin system -- Measurement of intracellular free calcium ion concentration in vascular smooth muscle cells: fluorescence imaging of cytosolic calcium / R.M. Touyz -- Measurement of phospholipase D activation in vascular smooth muscle cells / R.M. Touyz -- Measurement of cylic AMP and cyclic GMP in Xenopus oocytes stimulated with angiotensin II and atrial natriuretic factor / H. Ji -- Measurement of nitric oxide formation / C. Thorup -- Measuring renin secretion from juxtaglomerular cells / A. Kurtz -- pt. 7. Functional studies of the renin-angiotensin system -- Measurement of regional blood flow in the kidney using laser-Doppler flowmetry / R.J. Roman -- Measurement of renal tubular angiotensin II / J.D. Imig -- The in vitro blood-perfused juxtamedullary nephron technique / E.W. Inscho -- In vitro proximal tubule perfusion preparation / J.L. Garvin -- Isolated resistance artery preparation / J.P.M. Wesselman -- Measurement of vascular density / A.S. Greene -- Functional studies of the cerebral circulation / J.H. Lombard
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| Subject
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Angiotensins, Laboratory manuals
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| Subject
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Renin-Angiotensin System-- physiology
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Genetic Techniques
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Immunoassay
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Regional Blood Flow-- physiology
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| Dewey Classification
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612/.015756
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| LC Classification
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QP572.A54A545 2001
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| NLM classification
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2001 F-701
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WG 106A588 2001
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| Added Entry
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Wang, Donna H
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