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" High throughput protein expression and purification : "
edited by Sharon A. Doyle
Document Type
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BL
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Record Number
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602620
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Doc. No
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GBA896549b431839
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Title & Author
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High throughput protein expression and purification : : methods and protocols /\ edited by Sharon A. Doyle
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Publication Statement
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Totowa, N.J. :: Humana Press ;[London :: Springer, distributor],, c2009
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Series Statement
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Methods in molecular biology ;; 498
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Page. NO
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xii, 322 p. :: ill. (some col.) ;; 26 cm
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ISBN
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9781588298799 (hbk. : alk. paper)
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: 1588298795 (hbk. : alk. paper)
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: 9781597451963 (ebk.)
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: 1597451967 (ebk.)
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Bibliographies/Indexes
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Includes bibliographical references and index
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Contents
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High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.] -- Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery
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Subject
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Proteins-- Separation-- Methodology
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Subject
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Proteins-- isolation purification, Laboratory Manuals
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Dewey Classification
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572.636
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LC Classification
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QP551.H54 2009
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Added Entry
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Doyle, Sharon A., (Sharon Ann)
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