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" Antibody engineering : "


Document Type : BL
Record Number : 602902
Doc. No : b432121
Title & Author : Antibody engineering : : methods and protocols /\ edited by Patrick Chames
Edition Statement : 2nd ed
Series Statement : Methods in molecular biology,; 907
: Springer Protocols
Page. NO : xvi, 734 pages :: illustrations (some color) ;; 26 cm
ISBN : 9781617799730
: : 1617799734
: : 9781617799747
: : 1617799742
Bibliographies/Indexes : Includes bibliographical references and index
Contents : Use of IMGT® databases and tools for antibody engneering and humanization / Marie-Paule Lefranc ... [and others] -- Computer-assisted modeling of antibody variable domains / Oscar H.P. Ramos -- Cloning single-chain antibody fragments (scFv) from hyrbidoma cells / Lars Toleikis and André Frenzel -- Human in-cell scFv library from infiltrating B cell / Sylvie Peraldi-Roux -- Construction of human naive antibody gene libraries / Michael Hust ... [and others] -- Synthetic customized scFv libraries / Gautier Robin and Pierre Martineau -- Selection of stable scFv antibodies by phage display / Eeva-Christine Brockmann -- Generation of single domain antibody fragments derived from camelids and generation of manifold constructs / Cécile Vincke ... [and others] -- Generation and isolation of target-specific single-domain antibodies from shark immune repertoires / Mischa Roland ... [and others] -- Generation of human single domain antibody repertoires by Kunkel mutagenesis / Romain Rouet, Kip Dudgeon, and Daniel Christ -- Phage display and selections on purified antigens / Julie Matz and Patrick Chames -- Phage display and selections on cells / Klervi Even-Desrumeaux and Patrick Chames -- Humanization by CDR grafting and specificity-determining residue grafting / Jin Hong Kim and Hyo Jeong Hong -- Humanization by guided selections / Sang Jick Kim and Hyo Jeong Hong -- Selection of antibody fragments by yeast display / Nathalie Scholler -- Evolution of antibodies in vitro by ribosome display / Bryan M. Edwards and Mingyue He -- Mammalian cell surface display of full length IgG / Chen Zhou and Wenyan David Shen -- Production of antibody fragments in Escherichia coli / Tomohisa Katsuda ... [and others] -- Production of antibody derivatives in the methylotrophic yeast Pichia pastoris / Steve Schoonooghe, Jannick Leoen, and Jurgen Haustraete -- Monoclonal antibody expression in mammalian cells / Richard Yi Zhang and Wenyan David Shen -- Production of recombinant antibodies in Drosophila melanogaster S2 cells / Daniel X. Johansson, Thomas Krey, and Oskar Andersson -- Production of antibody fragments using the baculovirus-insect cell system / Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji -- Transient and stable expression of antibodies in Nicotiana species / Freydoun Garabagi, Michael D. McLean, and J. Christopher Hall -- Measuring antibody-antigen binding kinetics using surface plasmon resonance / Stephen Hearty, Paul Leonard, and Richard O'Kennedy -- Affinity determination of biotinylated antibodies by flow cytometry / Klervi Even-Desrumeaux and Patrick Chames -- Affinity maturation of antibodies : optimized methods to generate high-quality scFv libraries and isolate IgG candidates by high-throughput screening / Laurence Renaut ... [and others] -- Affinity maturation by semi-rational approaches / Rodrigo Barderas ... [and others] -- Molecular scanning : combining random mutagenesis, ribosome display, and bioinformatic analysis for protein engineering / Alfredo Darmanin-Sheehan ... [and others] -- Fucose-targeted glycoengineering of pharmaceutical cell lines / Christiane Ogorek ... [and others] -- Fc engineering : design, expression, and functional characterization of antibody variants with improved effector function / Stefanie Derer ... [and others] -- Fc engineering : serum half-life modulation through FcRn binding / Tove Olafsen -- Monoclonal antibody lead characterization : in vitro and in vivo methods / Axel Hernandez ... [and others] -- Production, purification, and characterization of scFv TNF ligand fusion proteins / Andrea Fick, Agnes Wyzgol, and Harald Wajant -- Antibody-IL2 fusion proteins for tumor targeting / Andreas A. Hombach and Hinrich Abken -- Recombinant immunotoxins with low endotoxins for clinical and animal studies / Masanori Onda -- Chimeric antigen receptors for T-cell based therapy / Eleanor J. Cheadle ... [and others] -- Selection and use of intracellular antibodies (intrabodies) / Sandrine Moutel, Clément Nizak, and Franck Perez -- Radiolabeled antibodies for cancer imaging and therapy / Jacques Barbet ... [and others] -- Design and production of multimeric antibody fragments, focused on diabodies with enhanced and clinical efficacy / Glenn A. Powers, Peter J. Hudson, and Michael P. Wheatcroft -- Production of bispecific antibodies : diabodies and tandem scFv / Nora Hornig and Aline Färber-Schwarz
Abstract : "Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more"--Publisher
Subject : Immunotechnology, Laboratory manuals
Subject : Antibodies-- therapeutic use, Laboratory Manuals
Subject : Antibodies-- genetics, Laboratory Manuals
Subject : Protein Engineering-- methods, Laboratory Manuals
LC Classification : ‭TP248.65.I49‬‭A58 2012‬
NLM classification : ‭W1‬‭ME9616J v.907 2012‬
: ‭QW 525‬
Added Entry : Chames, Patrick
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