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" Plant isoprenoids "
edited by Manuel Rodríguez-Concepción, Center for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain.
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BL
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| Record Number
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615014
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dltt
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| Title & Author
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Plant isoprenoids : methods and protocols /\ edited by Manuel Rodríguez-Concepción, Center for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain.
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| Publication Statement
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New York :: Humana Press,, [2014]
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| Series Statement
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Methods in molecular biology,; 1153
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Springer protocols
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| ISBN
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9781493906055
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: 1493906054
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9781493906062
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1493906062
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Plant isoprenoids: A General Overview -- Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts -- Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants -- Farnesyl Diphosphate Synthase Assay -- Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry -- Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity -- Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography -- Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry -- Analysis of Plant Polyisoprenoids -- Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots -- Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants -- Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry -- Isoprenoid and Metabolite Profiling of Plant Trichomes -- Sample Preparation for Single Cell Transcriptomics -- Essential Oil Glands in Citrus Fruit Peel as an Example -- Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions -- Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit -- Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS -- High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana -- Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens -- Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors -- A Flexible Protocol for Targeted Gene Co-Expression Network Analysis.
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| Subject
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Isopentenoids, Laboratory manuals.
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Terpenes-- metabolism, Laboratory Manuals.
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Plants-- metabolism, Laboratory Manuals.
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Terpenes-- analysis, Laboratory Manuals.
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Terpenes-- pharmacology, Laboratory Manuals.
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| Added Entry
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Rodríguez-Concepción, Manuel.
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Springer Science+Business Media.
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