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" T follicular helper cells "
edited by Marion Espéli, INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France, Michelle Linterman, Lymphocyte Signalling and Development ISP, Babraham Institute, Babraham Research Campus, Cambridge, UK.
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BL
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| Record Number
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615067
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dltt
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| Title & Author
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T follicular helper cells : methods and protocols /\ edited by Marion Espéli, INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France, Michelle Linterman, Lymphocyte Signalling and Development ISP, Babraham Institute, Babraham Research Campus, Cambridge, UK.
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| Publication Statement
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New York, NY :: Humana Press,, [2015]
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| Series Statement
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Methods in molecular biology,; 1291
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Springer protocols,
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| ISBN
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9781493924974
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: 1493924974
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9781493924981
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1493924982
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Includes bibliographical references and index.
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| Contents
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Identification of Mouse T Follicular Helper Cells by Flow Cytometry -- Identification of Follicular T Helper Cells in Tissue Sections -- Tracking Early T Follicular Helper Cell Differentiation In vivo -- Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination -- Retroviral Vector Expression in TCR Transgenic CD4+ T Cells -- Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells -- In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function -- Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation -- The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses -- Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205 -- Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry -- Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry -- In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses -- Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells -- Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential -- Analysis of Human Blood Memory T Follicular Helper Subsets -- Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood -- Quantifying Helper Cell Function of Human TFH Cells in vitro.
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| Abstract
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This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward. --
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Th1 cells-- Physiological effect, Laboratory manuals.
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Th2 cells-- Physiological effect, Laboratory manuals.
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T cells-- Physiological effect, Laboratory manuals.
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T-Lymphocytes, Helper-Inducer, Laboratory Manuals.
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| Added Entry
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Espéli, Marion.
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Linterman, Michelle.
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