| Document Type
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BL
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| Record Number
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622597
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| Doc. No
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dltt
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| Title & Author
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Laboratory methods in enzymology : : protein part D /\ edited by Jon R. Lorsch
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| Series Statement
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Methods in enzymology,; volume 559
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| Page. NO
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xi, 148 pages :: illustrations ;; 24 cm
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| ISBN
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0128002794
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: 9780128002797
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| Bibliographies/Indexes
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Includes bibliographical references and indexes
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| Contents
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Front Cover; Laboratory Methods in Enzymology: Protein Part D; Copyright; Contents; Contributors; Preface; Chapter One: Purification of His-Tagged Proteins; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Protocol A: Purification of His-tagged Proteins Under Native Conditions; 5.1. Tip; 6. Step 1A Preparation of a Cleared E. coli Lysate Under Native Conditions; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip; 6.7. Tip; 7. Step 2A Batch Purification of His-tagged Proteins from E. coli Under Native Conditions
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7.1. Overview7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 8. Step 1B Preparation of a Cleared E. coli Lysate Under Denaturing Conditions; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Tip; 8.6. Tip; 8.7. Tip; 9. Step 2B Batch Purification of His-tagged Proteins from E. coli Under Denaturing Conditions; 9.1. Overview; 9.2. Duration; 9.3. Tip; 9.4. Tip; 9.5. Tip; 9.6. Tip; References; Referenced Literature; Source References; Referenced Protocols in Methods Navigator
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Chapter Two: Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Equilibration of the Column; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step 2 Binding of the Protein Sample; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 7. Step 3 Removal of Unbound Proteins; 7.1. Overview; 7.2. Duration; 8. Step 4 Elution of the Bound Protein; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Tip; 8.6. Tip
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8.7. Tip8.8. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Chapter Three: Immunoaffinity Purification of Proteins; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 4.3. Tip; 4.4. Tip; 5. Step 1 Equilibrate Column; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step 2 Loading the Column; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Washing the Column; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Elution of the Protein from the Column; 8.1. Overview; 8.2. Duration; 8.3. Tip
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8.4. Tip8.5. Tip; 8.6. Tip; 9. Step 5 Column Regeneration and Storage; 9.1. Overview; 9.2. Duration; 9.3. Tip; References; Referenced Protocols in Methods Navigator; Chapter Four: Affinity Purification of Protein Complexes Using TAP Tags; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Preparation of Protein Lysates; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 2 Binding of the Protein A Tag to IgG-Sepharose; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip 7 Step 3 TEV Protease Cleave
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| Abstract
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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts
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| Subject
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Proteins-- Purification, Laboratory manuals
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| Subject
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Proteins-- Analysis, Laboratory manuals
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| Dewey Classification
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612.0151
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| LC Classification
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QP601.C733 vol. 559
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| Added Entry
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Lorsch, Jon
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