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" The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations / "
edited by Jennifer A. Doudna, Erik J. Sontheimer
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BL
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| Record Number
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622659
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| Doc. No
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dltt
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| Title & Author
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The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations /\ edited by Jennifer A. Doudna, Erik J. Sontheimer
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| Edition Statement
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First edition
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| Series Statement
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Methods in enzymology,; volume 546
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| Page. NO
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xx, 549 pages, 24 unnumbered pages of plates :: illustrations (some color) ;; 24 cm
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| ISBN
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9780128011850
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: 0128011858
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| Bibliographies/Indexes
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Includes bibliographical references and indexes
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| Contents
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In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases - Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Mutliplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function
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| Subject
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Genetic engineering, Laboratory manuals
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Genomics, Laboratory manuals
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Endonucleases, Laboratory manuals
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| Subject
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Restriction enzymes, DNA, Laboratory manuals
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| Dewey Classification
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660.6/5
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| LC Classification
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QP601.M49 v.546
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| Added Entry
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Doudna, Jennifer A.
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Sontheimer, Erik J.
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