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" Membrane proteins -- engineering, purification and crystallization / "
Edited by Arun K. Shukla
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BL
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| Record Number
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622773
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dltt
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| Title & Author
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Membrane proteins -- engineering, purification and crystallization /\ Edited by Arun K. Shukla
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| Series Statement
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Methods in enzymology,; volume 557
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| Page. NO
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xx, 624 pages, 18 unnumbered pages of plates :: illustrations (some color) ;; 24 cm
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| ISBN
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0128021837
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: 9780128021835
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| Bibliographies/Indexes
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Includes bibliographical references and indexes
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| Contents
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Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes -- A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs -- Membrane preparation and solubilization -- Amphipathic agents for membrane protein study -- Quantification of detergent using colorimetric methods in membrane protein crystallography -- Solubilizatoin of G protein-coupled receptors: a convenient strategy to explore lipid-receptor interaction -- Overexpression, isolation, purification, and crystallization of NhaA -- Purification, refolding, and cyrstallization of the outer membrane protein OmpG from Excherichia coli -- Biophysical approaches to the study of LeuT, a prokaryotic homolog of neurotransmitter sodium symporters -- Generation of recombinant antibody fragments for membrane protein crystallization -- Phage display selections for affinity reagents to membrane proteins in nanodiscs --Antibody fragments for stabilization and crystallization of G protein-coupled receptors and their signaling complexes -- Conformational analysis of G protein-coupled receptor signaling by hydrogen/deuterium exchange mass spectometry -- EPR studies of gating mechanisms in ion channels -- Magic-angle-spinning solid-state NMR of membrane proteins -- Solution NMR structure determination of polytopic a-helical membrane proteins: a guide to spin label paramagnetic relaxation enhancement restraints -- Inducing two-dimensional crystallization of membrane proteins by dialysis for electron crystallography -- Crystallization of membrane proteins by vapor diffusion -- Bicelles coming of age: an empirical approach to bicelle crystallization -- Fluorescence recovery after photobleaching in lipidic cubic phase (LCP-FRAP): a precrystallization assay for membrane proteins -- Crystallization of proteins from crude bovine rod outer segments -- Crystallization of photosystem II for time-resolved structural studies using an x-ray free electron laser -- Major intrinsic protein superfamily: channels with unique structural features and diverse selectivity filters -- Comparative sequence-function analysis of the major facilitator superfamily: the "mix-and-match" method -- Elucidating ligand-modulated conformational landscape of GPCRs using cloud-computing approaches
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| Subject
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Membrane proteins
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| Added Entry
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Shukla, Arun K.
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