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" Laboratory Methods in Enzymology : "
edited by Jon Lorsch
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BL
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| Record Number
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653063
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| Doc. No
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dltt
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| Main Entry
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Lorsch, Jon
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| Title & Author
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Laboratory Methods in Enzymology : : RNA /\ edited by Jon Lorsch
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| Series Statement
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Methods in enzymology,; v. 530
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| Page. NO
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lvii, 413 pages :: illustrations ;; 24 cm
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| ISBN
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9780124200371
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: 0124200370
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| Contents
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Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing
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| Subject
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Biotechnology-- Methodology
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| Subject
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Molecular biology-- Methodology
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| LC Classification
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QP601.C733
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