|
" Culture of animal cells : "
R. Ian Freshney.
| Document Type
|
:
|
BL
|
| Record Number
|
:
|
657363
|
| Doc. No
|
:
|
dltt
|
| Main Entry
|
:
|
Freshney, R. Ian.
|
| Title & Author
|
:
|
Culture of animal cells : : a manual of basic technique and specialized applications /\ R. Ian Freshney.
|
| Edition Statement
|
:
|
6th ed.
|
| Publication Statement
|
:
|
Hoboken, N.J. :: Wiley-Blackwell,, c2010.
|
| Page. NO
|
:
|
xxxi, 732 p., [28] p. of plates :: ill. (some col.) ;; 29 cm.
|
| ISBN
|
:
|
9780470528129 (cloth)
|
|
|
:
|
: 0470528125 (cloth)
|
| Bibliographies/Indexes
|
:
|
Includes bibliographical references and index.
|
| Contents
|
:
|
1. Introduction -- 2. Biology of cultured cells -- 3. Laboratory design, layout, and equipment -- 4. Equipment and materials -- 5. Aseptic technique -- 6. Safety, bioethics, and validation -- 7. Culture vessels and substrates -- 8. Defined media and supplements -- 9. Serum-free media -- 10. Preparation and sterilization -- 11. Primary culture -- 12. Subculture and cell lines -- 13. Cloning and selection -- 14. Cell separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation and immortalization -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized cells -- 23. Stem cells, germ cells, and amniocytes -- 24. Culture of tumor cells -- 25. Three-dimensional culture -- 26. Scale-up and automation -- 27. Specialized techniques -- 28. Training programs -- 29. Problem solving -- 30. In conclusion.
|
| Subject
|
:
|
Tissue culture, Laboratory manuals.
|
| Subject
|
:
|
Cell culture, Laboratory manuals.
|
| Subject
|
:
|
Cells, Cultured.
|
| Subject
|
:
|
Culture Techniques-- methods.
|
| Dewey Classification
|
:
|
571.6/381
|
| LC Classification
|
:
|
QH585.2.F74 2010
|
| NLM classification
|
:
|
2010 L-707
|
|
|
:
|
QU 300F885c 2010
|
| |