| Document Type
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BL
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| Record Number
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660998
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| Doc. No
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dltt
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| Title & Author
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Protocols for nucleic acid analysis by nonradioactive probes\ edited by Peter G. Isaac.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, c1994.
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| Series Statement
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Methods in molecular biology ;; v. 28
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| Page. NO
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xii, 268 p. :: ill. ;; 23 cm.
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| ISBN
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089603254X
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: 9780896032545
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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About nonradioactive nucleic acid detection -- Isolation of DNA from plants -- Isolation of high-molecular-weight DNA from animal cells -- Restriction enzyme digestion, gel electrophoresis and vacuum blotting of DNA to nylon membranes -- Isolation of plant RNA -- Isolation of total and polyA⁺RNA from animal cells -- Preparation of RNA gel blots -- Preparation of RNA dot blots -- Isolation of plasmids for the preparation of probes -- Production of hybridization probes by the PCR utilizing digoxigenin-modified nucleotides -- Production of DNA hybridization probes with digoxigenin-modified nucleotides by random hexanucleotide priming -- Digoxigenin labeling of RNA transcripts from multi- and single-locus DNA minisatellite probes -- Labeling of double-stranded DNA probes with biotin.
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(cont) Preparation of horseradish peroxidase-labeled probes -- Random prime labeling of DNA probes with fluorescein-11-dUTP -- Labeling of oligonucleotides with fluorescein -- Hybridization of digoxigenin-labeled probes to southern blots and detection by chemiluminescence -- Southern blot hybridization of digoxigenin-labeled RNA minisatellite probes and color detection -- Hybridization and detection of digoxigenin probes on RNA blots -- Hybridization of horseradish peroxidase-labeled probes and detection by enhanced chemiluminescence -- Hybridization and detection of fluorescein-labeled DNA probes using enhanced chemiluminescence -- Hybridization of fluorescein-labeled oligonucleotide probes and enhanced chemiluminescence detection.
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(cont) Preparation of chromosome spreads by root-tip meristem dissection for in situ hybridization with biotin-labeled probes -- Enzymatic treatment of plant material to spread chromosomes for in situ hybridization -- Use of biotin-labeled probes on plant chromosomes -- Direct fluorochrome-labeled DNA probes for direct fluorescent in situ hybridization to chromosomes -- Detection of dioxigenin-labeled DNA probes hybridized to plant chromosomes in situ -- Preparation of tissue sections and slides for mRNA hybridization -- Detecting mRNA in tissue sections with digoxigenin-labeled probes -- Detection of mRNA in whole mounts of mouse embryos using digoxigenin riboprobes -- PACE (Probe Assay-Chemiluminescence Enhanced): a magnetic bead assay for noncultural diagnosis of gonorrhea.
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(cont) Detection of foodborne pathogens using DNA probes and a dipstick format -- Analysis of gene sequences by hybridization of PCR-amplified DNA to covalently bound oligonucleotide probes: the reverse dot blot method -- RAPD assay: a novel technique for genetic diagnostics -- Nonradioactive oligonucleotide probes for detecting products of the ligase chain reaction -- Nucleic acid sequence-based amplification (NASBA).
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| Subject
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Nucleic acid probes.
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| Subject
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Nucleic acids-- Analysis.
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| Added Entry
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Isaac, Peter G.
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| Parallel Title
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Nonradioactive probes
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