| Document Type
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BL
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| Record Number
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661032
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| Doc. No
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dltt
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| Title & Author
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Genomic imprinting : methods and protocols /\ edited by Andrew Ward.
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| Publication Statement
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Totowa, NJ :: Humana Press,, c2002.
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| Series Statement
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Methods in molecular biology ;; v. 181
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| Page. NO
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xii, 377 p. :: ill. ;; 24 cm.
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| ISBN
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089603741X (alk. paper)
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: 9780896037410 (alk. paper)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro.
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methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants.
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| Subject
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Genomic imprinting, Laboratory manuals.
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| Added Entry
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Ward, Andrew,1964-
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