| Document Type
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BL
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| Record Number
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661033
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| Doc. No
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dltt
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| Title & Author
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Antigen processing and presentation protocols\ edited by Joyce C. Solheim.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, c2001.
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| Series Statement
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Methods in molecular biology ;; 156
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| Page. NO
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xiii, 281 p. :: ill. ;; 24 cm.
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| ISBN
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0896037452 (alk. paper)
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: 9780896037458 (alk. paper)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Purification of 20S proteasomes -- Use of proteasome inhibitors to examine processing of antigens for major histocompatibility complex class I presentation -- Tracing the route taken by peptides and major histocompatibility complex class I molecules in presentation of exogenous antigens -- Functional analysis of antigen processing and major histocompatibility complex class II-restricted presentation -- Quantitation of major histocompatibility complex antigen endocytosis using capture ELISA -- In vitro generation of functional human and murine dendritic cells -- Genetic engineering of dendritic cells using retrovirus-based gene transfer techniques -- Use of vaccinia virus expression vectors to investigate antigen processing and presentation -- Sindbis virus-based vectors for the study of class I antigen presentation in vitro and in vivo -- Precision genotyping of human leukocyte antigen-A, -B, and -C loci via direct DNA sequencing -- Photolabeling the transporter associated with antigen processing -- Application of one-dimensional isoelectric focusing to separate different major histocompatibility complex class I alleles and determine their allelic interactions with transporter associated with antigen processing (TAP) -- Analysis of major histocompatibility complex class I interactions with endoplasmic reticulum proteins -- Methods of purification of heat shock protein-peptide complexes for use as vaccines against cancers and infectious diseases -- Random peptide libraries: a tool for analyzing peptide specificity of major histocompatibility complex class I molecules -- Neural network method for predicting peptides that bind major histocompatibility complex molecules -- Analysis of T-cell response using altered peptide ligands -- Assays of thymic selection: fetal thymus organ culture and in vitro thymocyte dulling assay -- Protocol for diversion of a CD4+ response to the T-Helper 2 cell pathway -- Quantitating presentation of MHC class I-restricted antigens -- Identification of CD8+ T-cell-stimulating antigen genes in cDNA libraries -- Generation of antigen-specific, lacZ-inducible T-cell hybrids.
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| Subject
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Antigen presenting cells, Laboratory manuals.
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| Subject
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Antigens, Laboratory manuals.
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| Subject
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HLA histocompatibility antigens, Laboratory manuals.
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| Subject
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Major histocompatibility complex, Laboratory manuals.
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| Subject
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Molecular immunology, Laboratory manuals.
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| Added Entry
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Solheim, Joyce C.
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