| Document Type
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BL
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| Record Number
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661040
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| Doc. No
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dltt
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| Title & Author
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Gene probes : principles and protocols /\ edited by Marilena Aquino de Muro and Ralph Rapley.
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| Publication Statement
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Totowa, NJ :: Humana Press,, c2002.
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| Series Statement
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Methods in molecular biology ;; 179
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| Page. NO
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xi, 272 p. :: ill. (some col.) ;; 24 cm.
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| ISBN
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0896038858 (alk. paper)
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: 9780896038851 (alk. paper)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Target format and hybridization conditions -- End labeling procedures: an overview -- Photobiotin labeling -- Nick translation -- Random primed labeling -- Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence In Situ hybridization -- Evaluation of alternations in the tumor suppressor genes INK4A and INK4B in human bladder tumors -- The [beta]-tubulin gene region as a molecular marker to distinguish Leishmania parasites -- Detection of malignant plasma cells in the bone marrow and peripheral blood of patients with multiple myeloma -- Detection of Chlamydia trachomatis by DNA amplification -- Isolation and identification of Campylobacter species by use of selective enrichment, nucleic acid amplification methods, and gene probes -- Diagnosis of human papillomavirus using In Situ hybridization and In Situ polymerase chain reaction -- Quantitation of In Situ hybridization using image analysis of radioactively labeled RNA probes -- Stable isotope probing for detection of methanotrophs after enrichment with ¹³CH₄ -- Detection of Enterococci in freshwater and seawater (16S and 23S rRNA Enterococci Oligonucleotide Probes) -- Analysis of 16S-23S rRNA intergenic spacer of Vibrio cholerae and Vibrio mimicus for detection of these species -- Colony hybridization of bacterial isolates with Burkholderia cepacia-specific probes -- In Situ hybridization of Burkholderia cepacia cells -- Detection of polychlorinated biphenyl-degrading organisms in soil -- Detection of single-copy genes in DNA from transgenic plants -- Differentiation between transcripts of genes belonging to small families during fruit ripening and abscission -- Polymerase chain reaction detection of nvasive Shigella and Salmonella enterica in food -- Polymerase chain reaction for detection of Listeria monocytogenes.
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| Subject
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Nucleic acid probes, Laboratory manuals.
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| Added Entry
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Aquino de Muro, Marilena.
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Rapley, Ralph.
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