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" Generation of cDNA libraries "
edited by Shao-Yao Ying.
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BL
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| Record Number
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668844
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dltt
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| Title & Author
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Generation of cDNA libraries : methods and protocols /\ edited by Shao-Yao Ying.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, c2003.
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| Series Statement
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Methods in molecular biology ;; v. 221
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| Page. NO
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xiii, 335 p. :: ill. ;; 24 cm.
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| ISBN
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1588290662 (alk. paper)
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: 9781588290663 (alk. paper)
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: 1592593593 (EBOOK)
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: 9781592593590 (EBOOK)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz ... [et al.] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jürgen Fülle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT( - )) cDNA for gene identification / San Ming Wang ... [et al.] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen ... [et al.] -- Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva ... [et al.] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying.
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| Subject
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Antisense DNA, Laboratory manuals.
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| Added Entry
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Ying, Shao-Yao.
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