| Document Type
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BL
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| Record Number
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668845
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| Doc. No
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dltt
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| Title & Author
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Protein purification protocols
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| Edition Statement
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Second edition / edited by Paul Cutler
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| Series Statement
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Methods in molecular biology ;; v. 244
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| Page. NO
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xii, 484 pages :: illustrations ;; 26 cm
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| ISBN
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1588290670
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: 9781588290670
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: 159259655X
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: 9781592596553
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| Bibliographies/Indexes
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Includes bibliographical references and index
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| Contents
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General strategies / S. Doonan and P. Cutler -- Preparation of extracts from animal tissues / J.M. Skehel -- Protein extraction from plant tissues / R.J. Fido [and others] -- Extraction of recombinant protein from bacteria / A.F. McGettrick and D.M. Worrall -- Protein extraction from fungi / P.D. Bridge, T. Kokubun and M.S. Simmonds -- Subecllular fractionation of animal tissues / N.M. Ryan -- Subcellular fractionation of plant tissues: isolation of plastids and mitochondria / A.K. Tobin and C.G. Bowsher -- The extraction of enzymes from plant tissues rich in phenolic compounds / W.S. Pierpoint -- Avoidance of proteolysis in extracts / R.J. Beynon and S. Oliver -- Concentration of extracts / S. Doonan -- Making and changing buffers / S. Doonan -- Purification and concentration by ultrafiltration / P. Schratter -- Bulk purification by fractional precipitation / S. Doonan -- Ion-exchange chromatography / C. Selkirk -- Hydrophobic interaction chromatography / P.A. O'Farrell -- Affinity chromatography / P. Cutler -- Dye-ligand affinity chromatography / A.F. McGettrick and D.M. Worrall -- Lectin affinity chromatography / I. West and O. Goldring -- Immunoaffinity chromatography / P. Cutler -- Immobilized metal-ion affinity chromatography / T.T. Yip and T.W. Hutchens -- Chromatography on hydroxyapatite / S. Doonan -- Thiophilic affinity chromatography and related methods / P. Matejtschuk -- Affinity precipitation methods / J.A. Irwin and K.F. Tipton -- Isoelectric focusing / R. Westermeier -- Chromatofocusing / T.J. Mantle and P. Noone -- Size-exclusion chromatography / P. Cutler -- Fast protein liquid chromatography / D. Sheehan and S. O'Sullivan -- Reversed-phase chromatography of proteins / W.A. Neville -- Extraction of membrane proteins / K. Ohlendieck -- Removal of detergent from protein fractions / K. Ohlendieck -- Purification of membrane proteins / K. Ohlendieck -- Lyophilization of proteins / C. O'Fagain -- Storage of pure proteins / C. O'Fagain -- Electroelution of proteins from polyacrylamide gels / M.J. Dunn -- Electroblotting of proteins from polyacrylamide gels / M.J. Dunn -- Two-dimensional polyacrylamide gel electrophoresis for proteome analyses / N.A. Jones -- Microscale solution isoelectrofocusing: a sample prefractionation method for comprehensive proteome analysis / X. Zuo and D.W. Speicher -- Practical column chromatography / S. Doonan -- Detection methods / J. Mozdzanowski and S. Burman -- Peptide proteomics / D.E. McNulty and J.R. Slemmon -- Multidimensional liquid chromatography of proteins / R. Watson and T. Nadler -- Mass spectrometry / D.J. Bell -- Purification of therapeutic proteins / J. Bonnerjea -- Purification process scale-up / K. Prince and M. Smith
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| Abstract
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In this new edition, the author completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. It brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and thie functions today
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| Subject
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Proteins-- Purification, Laboratory manuals
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| Subject
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Proteins-- isolation purification, Laboratory Manuals
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| Subject
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Proteins-- Isolation purification, Laboratory manuals
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| Dewey Classification
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572.645
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547.7/5046
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| LC Classification
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QP551.P69756 2004
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| NLM classification
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W1ME9616J v.244 2004
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QU 25P967 2004
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| Added Entry
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Cutler, Paul,1962-
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