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" Avidin-biotin interactions : "
edited by Robert J. McMahon.
Document Type
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BL
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Record Number
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668908
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Doc. No
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dltt
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Title & Author
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Avidin-biotin interactions : : methods and applications /\ edited by Robert J. McMahon.
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Publication Statement
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Totowa, NJ :: Humana Press ;[London :: Springer, distributor],, c2008.
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Series Statement
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Methods in molecular biology,; 418.
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Page. NO
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xiii, 232 p. :: ill. ;; 23 cm.
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ISBN
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9781588295835 (hbk.)
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: 1588295834 (hbk.)
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: 9781597455794 (e-ISBN)
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: 1597455792 (e-ISBN)
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Bibliographies/Indexes
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Includes bibliographical references and index.
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Contents
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Preparation of avidin conjugates / Rosaria P. Haugland and Mahesh K. Bhalgat -- Coupling of antibodies with biotin / Rosaria P. Haugland and Wendy W. You -- Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and strptavidin / Sara R. Zwart and Brandon J. Lewis -- Use of the avidin (Imino) biotin system as a general approach to affinity precipitation / Ruth Freitag and Frank Hilbrig -- Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis / Bao-Shiang Lee ... [et al.] -- Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein / Nicolas Humbert and Thomas R. Ward -- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives / Andreas Ebner ... [et al.] -- quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins / Christoph Rösli ... [et al.] -- High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli / Nicolas Humbert ... [et al.] -- Endogenous biotin in rat brain : implications for false-positive results with avidin-biotin and streptavidin-biotin techniques / Bruce E. McKay, Michael L. Molineux, and Ray W. Turner -- Pseudo-immunolabelling with the avidin-biotin-peroxidase complex due to the presence of endogenous biotin in the retina / Willem Kamphuis and Jan Klooster -- Use of synthetic peptides for identifying biotinylation sites in human histones / Gabriela Camporeale ... [et al.] -- Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay / Kirsten Liebert and Albert Jeltsch -- Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin / Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux -- Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagnostic procedure / Karel Smetana, Jr. and Sabine André -- A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin / Krista McCutcheon -- Biotin-protein bond : instability and structural modification to provide stability for in vivo applications / Donald M. Mock and Anna Bogusiewicz.
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Subject
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Biotin.
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Subject
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Avidin.
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Subject
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Affinity chromatography.
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Subject
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Affinity electrophoresis.
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LC Classification
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QP552.A94A95
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Added Entry
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McMahon, Robert Joseph,1958-
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