| Document Type
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BL
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| Record Number
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685102
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| Doc. No
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b507291
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| Title & Author
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Recombinant protein production in yeast : : methods and protocols /\ edited by Roslyn M. Bill.
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| Publication Statement
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New York :: Humana Press,, c2012.
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| Series Statement
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Methods in molecular biology,; 866
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Springer protocols
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| Page. NO
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xii, 248 p. :: ill. (some col.) ;; 27 cm.
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| ISBN
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1617797693
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: 9781617797699
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby ... [et al.] -- Preparation of Pichia pastoris expression plasmids / Christel Logez ... [et al.] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora ... [et al.] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby.
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| Subject
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Recombinant proteins, Laboratory manuals.
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Yeast fungi-- Biotechnology, Laboratory manuals.
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Recombinant Proteins-- biosynthesis, Laboratory Manuals.
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Yeasts, Laboratory Manuals.
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| Subject
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Pichia, Laboratory Manuals.
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| Subject
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Saccharomyces cerevisiae, Laboratory Manuals.
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| Added Entry
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Bill, Roslyn M.,1967-
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