|
" Protein chromatography : "
edited by Dermot Walls and Sinéad T. Loughran.
| Document Type
|
:
|
BL
|
| Record Number
|
:
|
685267
|
| Doc. No
|
:
|
b507456
|
| Title & Author
|
:
|
Protein chromatography : : methods and protocols /\ edited by Dermot Walls and Sinéad T. Loughran.
|
| Publication Statement
|
:
|
New York, N.Y. :: Humana Press,, c2011.
|
| Series Statement
|
:
|
Methods in molecular biology,; v. 681
|
|
|
:
|
Springer protocols
|
| Page. NO
|
:
|
x, 527 p. :: ill. (some col.) ;; 27 cm.
|
| ISBN
|
:
|
1607619121
|
|
|
:
|
: 9781607619123
|
| Bibliographies/Indexes
|
:
|
Includes bibliographical references and index.
|
| Contents
|
:
|
A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.
|
| Subject
|
:
|
Chromatographic analysis.
|
| Subject
|
:
|
Proteins-- Analysis.
|
| Subject
|
:
|
Proteomics.
|
| Subject
|
:
|
Chromatography-- methods.
|
| Subject
|
:
|
Proteins-- analysis.
|
| Added Entry
|
:
|
Loughran, Sinéad T.
|
|
|
:
|
Walls, Dermot.
|
| |