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" DNA damage detection in situ, ex vivo, and in vivo : "
edited by Vladimir V. Didenko.
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BL
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| Record Number
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685345
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b507534
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| Title & Author
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DNA damage detection in situ, ex vivo, and in vivo : : methods and protocols /\ edited by Vladimir V. Didenko.
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| Publication Statement
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New York ;London :: Springer,, c2011.
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| Series Statement
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Methods in molecular biology ;; v. 682
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Springer protocols
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xiii, 313 p. :: ill. (some col.) ;; 27 cm.
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| ISBN
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1603274081
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: 9781603274081
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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In situ detection of apoptosis by the TUNEL assay: An overview of techniques -- Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage -- EM-ISEL: A useful tool to visualize DNA damage at the ultrastructural level -- In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase -- In situ ligation: A decade and a half of experience -- In situ ligation simplified: Using PCR fragments for detection of double-strand DNA breaks in tissue sections -- 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach -- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry -- Fluorochrome-labeled inhibitors of caspases: Convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis -- Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair -- Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) -- Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells -- Ultrasound imaging of apoptosis: Spectroscopic detection of DNA-damage effects at high and low frequencies -- Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)p-postlabeling and immunohistochemistry -- ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells -- Cytokinesis-block micronucleus cytome assay in lymphocytes -- Buccal micronucleus cytome assay -- [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin -- Immunologic detection of benzo(a)pyrene-DNA adducts -- Non-invasive assessment of oxidatively damaged DNA: Liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine -- Assessing sperm DNA fragmentation with the sperm chromatin dispersion test.
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| Subject
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DNA damage-- Research-- Methodology.
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DNA damage.
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DNA-- Analysis.
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DNA Damage.
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DNA-- analysis.
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Microbiological Techniques, Laboratory Manuals.
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| Added Entry
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Didenko, Vladimir V.
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