|
" Guidelines for molecular analysis in archive tissues / "
Giorgio Stanta, editor.
| Document Type
|
:
|
BL
|
| Record Number
|
:
|
690635
|
| Doc. No
|
:
|
b512824
|
| Title & Author
|
:
|
Guidelines for molecular analysis in archive tissues /\ Giorgio Stanta, editor.
|
| Publication Statement
|
:
|
Heidelberg ;New York :: Springer,, 2011.
|
| Page. NO
|
:
|
1 online resource (xvi, 323 pages) :: illustrations (some color)
|
| ISBN
|
:
|
3642178901
|
|
|
:
|
: 9783642178900
|
|
|
:
|
3642178898
|
|
|
:
|
9783642178894
|
| Notes
|
:
|
Title from PDF t.p. (SpringerLink, viewed Jun 13, 2011)
|
| Bibliographies/Indexes
|
:
|
Includes bibliographical references and index
|
| Contents
|
:
|
Archive Tissues -- Preanalytical Time Interval (PATI) and Fixation -- Formalin-Free Fixatives -- Manual Microdissection -- Tissue Microarray (TMA) -- Laser Capture Microdissection (LCM) -- DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues -- DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells) -- Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- DNA Extraction from Blood and Forensic Samples -- Restoration and Reconstruction of DNA Length -- RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues -- RNA Temperature Demodification -- MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- Quantification of Nucleic Acids -- Integrity Assessment of Nucleic Acids -- DNase Treatment of RNA -- Reverse Transcription -- General Protocol for End-Point PCR -- Quantitative End-Point PCR -- Nested-PCR -- General Protocol for Dot-Blot -- PCR-ELISA -- Quantitative Real-Time RT-PCR -- DNA Sequencing from PCR Products -- Pyrosequencing Analysis for Detection of KRAS Mutation -- Microsatellite Instability (MSI) Detection in DNA from FFPE Tissues -- General Protocol for Loss of Heterozygosity Detection -- Qualitative Methylation Status Assessment -- Quantitative Methylation Status Assessment in DNA from FFPE Tissues with Bisulfite Modification and Real-Time Quantitative MSP -- Comparative Genomic Hybridisation (CGH) -- Multiplex Ligation-dependent Probe Amplification (MLPA) -- Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections -- Introduction on Genome-wide Expression Profiling from Formalin-Fixed Paraffin-Embedded Tissues Using Microarrays -- Introduction -- Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- Rapid Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue Samples -- Protein Extraction Protocol from Tissues Treated with Formalin-Free Fixatives -- Trichloroacetic Acid (TCA) Precipitation of Proteins -- One-Dimensional Sodium-Dodecyl-Sulfate (SDS) Polyacrylamide Gel Electrophoresis -- Visualization of Proteins -- Western Blotting -- Dot Blot -- Membrane Stripping -- Reverse-Phase Protein Microarrays -- Introduction -- Sample Preparation and In Gel Tryptic Digestion for Mass Spectrometry Experiments -- MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissues -- Introduction -- Internal Quality Control (IQC) Organization -- Standards for Equipment and Reagents
|
| Subject
|
:
|
Pathology, Molecular.
|
| Dewey Classification
|
:
|
616.07
|
| LC Classification
|
:
|
RB113.G85 2011
|
| Added Entry
|
:
|
Stanta, Giorgio.
|
| Added Entry
|
:
|
Ohio Library and Information Network.
|
| |