Document Type
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BL
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Record Number
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691826
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Doc. No
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b514015
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Title & Author
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DNA typing protocols : : molecular biology and forensic analysis /\ Bruce Budowle ... [and others]
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Publication Statement
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Natick, MA :: Eaton Pub.,, [2000]
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, ©2000
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Page. NO
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vii, 304 pages :: illustrations ;; 26 cm
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ISBN
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1881299236
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Notes
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"A BioTechniques books publication."
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Bibliographies/Indexes
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Includes bibliographical references and index
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Contents
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1. Introduction -- 2. Sample collection and storage -- 3. Extraction of DNA -- 4. Determination of the quantity of DNA -- 5. Restriction fragment-length polymorphism typing of variable number tandem repeat loci -- 6. PCR-based analyses: allele-specific oligonucleotide assays -- 7. Electrophoretic separation and allele detection by silver staining for the loci D1S80 and amelogenin -- 8. Short tandem repeat loci typing using polyacrylamide gel electrophoresis and decoupled fluorescent scanner detection -- 9. Capillary electrophoresis for short tandem repeat loci typing and real time fluorescent detection -- 10. PCR-based analysis: mitochondrial DNA sequencing -- Appendix 1. Quality assurance standards -- Appendix 2. Allele frequency data -- Appendix 3. Reagents and supplies
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Subject
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DNA fingerprinting, Laboratory manuals
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Subject
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DNA-- Analysis, Laboratory manuals
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Subject
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Forensic genetics, Laboratory manuals
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Subject
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Polymerase chain reaction, Laboratory manuals
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LC Classification
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RA1057.5.D534 2000
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Added Entry
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Budowle, Bruce,1953-
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