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" Molecular methods for evolutionary genetics / "
edited by Virginie Orgogozo, Matthew V. Rockman
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BL
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| Record Number
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693938
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b516127
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| Title & Author
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Molecular methods for evolutionary genetics /\ edited by Virginie Orgogozo, Matthew V. Rockman
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| Publication Statement
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New York :: Humana Press,, [2011]
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, ©2011
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| Series Statement
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Methods in molecular biology,; 772
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Springer protocols
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| Page. NO
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xvi, 503 p. :: ill. (some col.) ;; 27 cm
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| ISBN
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1617792276 (alk. paper)
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: 9781617792274 (alk. paper)
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1617792284 (e-ISBN)
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9781617792281 (e-ISBN)
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| Bibliographies/Indexes
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Includes bibliographical references and index
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| Contents
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Characterizing cDNA ends by circular RACE / Patrick T. McGrath. Identification of DNA sequences that flank a known region by inverse PCR / Anastasios Pavlopoulos -- Analyzing candidate gene transcripts. Quantification of transcript levels with quantitative RT-PCR / Karen L. Carleton. Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences / Patricia J. Wittkopp. Whole-mount in situ hybridization of sectioned tissues of species hybrids to detect cis-regulatory changes in gene expression pattern / Ryo Futahashi. Identifying fluorescently labeled single molecules in image stacks using machine learning / Scott A. Rifkin -- Testing candidate genes and candidate mutations. Experimental approaches to evaluate the contributions of candidate cis-regulatory mutations to phenotypic evolution / Mark Rebeiz and Thomas M. Williams. Experimental approaches to evaluate the contributions of candidate protein-coding mutations to pheotypic evolution / Jay F. Storz and Anthony J. Zera. Making reporter gene constructs to analyze cis-regulatory elements / José Bessa and José Luis Gómez-Skarmeta. PCR directed in vivo plasmid construction using homologous recombination in baker's yeast / Erik C. Andersen. Production of fosmid genomic libraries optimized for liquid culture recombineering and cross-species transgenesis / Radoslaw Kamil Ejsmont ... [et al.] Recombination-mediated genetic engineering of large genomic DNA transgenes / Radoslaw Kamil Ejsmont ... [et al.] Overlap extension PCR : an efficient method for transgene construction / Matthew D. Nelson and David H.A. Fitch. Gene knockdown analysis by double-stranded RNA injection / Benjamin N. Philip and Yoshinori Tomoyasu
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Characterizing the genome. Genome size determination using flow cytometry of propidium iodide-stained nuclei / Emily E. Hare and J. Spencer Johnston. Chromosome analysis in invertebrates and vertebrates / David M. Rowell, Shu Ly Lim, and Frank Grutzner. Genomic libraries : I. Constuction and screening of fosmid genomic libraries / Mike A. Quail ... [et al.] Genomic libraries : II. Subcloning, sequencing, and assembling large-insert genomic DNA clones / Mike A. Quail ... [et al.] -- Targeting regions of the genome. Reduced representation methods for subgenomic enrichment and next-generation sequencing / Jeffrey M. Good. Accessing the transcriptome : how to normalize mRNA pools / Heiko Vogel and Christopher W. Wheat. Transcriptome sequencing goals, assembly, and assessment / Christopher W. Wheat and Heiko Vogel. Rapid retrieval of DNA target sequences by primer extension capture / Adrian W. Briggs -- Measuring genetic diversity. SNP discovery and genotyping for evolutionary genetics using RAD sequencing / Paul D. Etter ... [et al.] DNA microarray-based mutation discovery and genotyping / David Gresham. Genotyping with Sequenom / Martina Bradić, João Costa, and Ivo M. Chelo. Isolating microsatellite loci : looking back, looking ahead / José A. Andrés and Steven M. Bogdanowicz. Design of custom oligonucleotide microarrays for single species or interspecies hybrids using array olico selector / Amy A. Caudy -- Obtaining candidate gene sequences. Identification of homologous gene sequences by PCR with degenerate primers / Michael Lang and Virginie Orgogozo
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| Subject
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Evolutionary genetics, Laboratory manuals
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Biological Evolution, Laboratory Manuals
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Genetic Variation, Laboratory Manuals
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| Dewey Classification
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572.8/38
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| LC Classification
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QH390.M65 2011
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QH390.M65 2011
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| NLM classification
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W1ME9616J v.772 2011
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QU 25
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| Added Entry
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Orgogozo, Virginie
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Rockman, Matthew V
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