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" Immunocytochemical methods and protocols "
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BL
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| Record Number
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695192
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| Doc. No
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b517381
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| Title & Author
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Immunocytochemical methods and protocols
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| Edition Statement
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3rd ed. // edited by Constance Oliver and Maria Célia Jamur
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| Publication Statement
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New York, NY :: Humana Press,, c2010
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| Series Statement
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Methods in molecular biology,; v. 588
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Springer protocols
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| Page. NO
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xii, 416 p. :: ill. ;; 27 cm
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| ISBN
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1588294633 (alk. paper)
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: 9781588294630 (alk. paper)
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| Notes
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Previous ed.: 1999
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| Bibliographies/Indexes
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Includes bibliographical references and index
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| Contents
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Overview of antibodies for immunochemistry / Constance Oliver and Maria Célia Jamur -- Introduction to the purification of antibodies / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ammonium sulfate fractionation or gel filtration / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ion-exchange chromatography / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : affinity chromatography--Protein A and Protein G Sepharose / Ana Cristina Grodzki and Elsa Berenstein -- Conjugation of fluorochromes to antibodies / Su-Yau Mao and J. Michael Mullins -- Biotinylation of antibodies / Su-Yau Mao -- Cell fixatives for immunostaining / Maria Célia Jamur and Constance Oliver -- Permeabilization of cell membranes / Maria Célia Jamur and Constance Oliver -- Preparation of frozen sections for analysis / Gary L. Bratthauer -- Processing of cytological specimens / Gary L. Bratthauer -- Processing of tissue culture cells / Gary L. Bratthauer -- Processing of tissue specimens / Gary L. Bratthauer -- Heat induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections / Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, and Mark Raffeld -- Fluorochromes : properties and characteristics / J. Michael Mullins -- Direct immunofluorescent labeling of cells / Maria Veronica Dávila Pástor -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescent visualization of macromolecules in Drosophila whole mounts / Ricardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto Moda -- Overview of conventional fluorescence photomicrography / J. Michael Mullins -- Overview of confocal microscopy / William D. Swaim -- Overview of laser microbeam applications as related to antibody targeting / P. Scott Pine -- Immuno-laser capture microdissection of rat brain neurons for real time quantitative PCR / Denis G. Baskin and L. Scot Bastian -- Overview of antigen detection through enzymatic activity / Gary L. Bratthauer -- The peroxidase-antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- The avidin-biotin complex (ABC) method and other avidin-biotin binding methods / Gary L. Bratthauer -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue / Mark Raffeld and Elaine S. Jaffe -- Multiple antigen immunostaining procedures / Tibor Krenacs, Laszlo Krenacs, and Mark Raffeld -- Immunoenzymatic quantitative analysis of antigens expressed on the cell surface (cell-ELISA) / Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira -- Use of immunogold with silver enhancement / Constance Oliver -- Overview of flow cytometry and fluorescent probes for flow cytometry / Robert E. Cunningham -- Tissue disaggregation / Robert E. Cunningham -- Indirect immunofluorescent labeling of viable cells / Robert E. Cunningham -- Indirect immunofluorescent labeling of fixed cells / Robert E. Cunningham -- Fluorescent labeling of DNA / Robert E. Cunningham -- Deparaffinization and processing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver and Maria Célia Jamur -- Preparation of colloidal gold / Constance Oliver -- Conjugation of colloidal gold to proteins / Constance Oliver -- Colloidal gold/streptavidin methods / Constance Oliver -- Pre-embedding labeling methods / Constance Oliver -- Postembedding labeling methods / Constance Oliver -- The Clinical immunohistochemistry laboratory : regulations and troubleshooting guidelines / Patricia A. Fetsch and Andrea Abati
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| Subject
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Immunocytochemistry, Laboratory manuals
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In situ hybridization, Laboratory manuals
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| Subject
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Immunohistochemistry-- methods
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Immunohistochemistry-- standards
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| Dewey Classification
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571.96
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| LC Classification
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QR187.I45
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| Added Entry
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Jamur, Maria Célia
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Oliver, Constance
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