| Document Type
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BL
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| Record Number
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698343
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b520532
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| Title & Author
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Transgenesis techniques : : principles and protocols /\ edited by David Murphy, David A. Carter.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, [1993]
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, ©1993
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| Series Statement
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Methods in molecular biology ;; v. 18
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| Page. NO
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xii, 467 pages :: illustrations ;; 23 cm.
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| ISBN
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0896032450 (pbk.)
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: 9780896032453 (pbk.)
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| Bibliographies/Indexes
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Includes bibliographical references and index
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| Contents
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(cont) Genomic analysis of transgenic animals: southern blotting -- Expression analysis of transgenic animals -- Preparation of RNa from the tissues of transgenic animals -- Northern blotting -- RNA slot blotting -- Analysis of gene expression by PCR -- Nuclear run-on analysis of transcription -- S1 nuclease protection mapping -- Primer extension -- In situ hybridization analysis of transgenic tissues -- Immunohistochemical analysis of transgene expression -- Postmortem examination of transgenic mice -- Histological examination of transgenic mice -- Reporter enzyme assays -- Appendix -- Restriction endonuclease digestion of DNA -- Agarose gel electrophoresis -- Working with radioactivity in the transgenic animal laboratory -- Random primed labeling of DNA -- Filter hybridization -- DNA transfection
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Introduction and topical reviews -- Introduction to transgenesis -- Transgenic rodents and the study of the central nervous system -- Transgenic animals and the study of cancer -- Transgenic animals and the study of the immune system -- Commercial and agricultural applications of animal transgenesis -- Transgenesis in invertebrate and lower vertebrate species -- P element-mediated germ-line transformation of Drosophila -- Gene transfer by microinjection in the zebra fish Brachydanio rerio -- Transgenic induction in Salmonid and Tilapia fish -- Transgenesis in the mouse -- Overview of transgenic mouse production -- Transgene design -- Isolation of DNA fragments for microinjection -- Establishing a colony for efficient production of transgenic mice -- Mating mice -- Anesthetizing mice -- Vasectomizing a mouse -- Preparation of culture media for fertilized one-cell mouse eggs -- Collection of fertilized one-cell mouse eggs for microinjection
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Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs I: manual injection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs II: automatic injection -- Delivery of microinjected eggs to surrogate mothers by oviduct transfer -- Caesarean section and fostering -- In vitro fertilization of mouse eggs -- In vitro isolation of murine embryonic stem cells -- Production of chimeras derived from murine embryonic stem cells -- Cryopreservation of transgenic mouse lines -- Transgenesis in the rat -- Introduction to the physiology and husbandry of the rat -- Mating of rats -- Vasectomy of the male rat -- Harvest of fertilized one-cell rat eggs -- Implantation of microinjected eggs -- Cryopreservation of transgenic rat lines -- Transgenesis in a domestic species: the sheep -- Production of transgenic sheep -- Cryopreservation of transgenic sheep lines -- Genomic analysis of transgenic animals -- Isolation of genomic DNA from tail tissue -- Slot blotting of genomic tail DNA -- Genomic analysis of transgenic animals by the polymerase chain reaction
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| Subject
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Animal genetic engineering-- Technique.
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| Subject
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Genetic transformation-- Technique.
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| Subject
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Transgenic animals.
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| Added Entry
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Carter, David A., (David Allan),1954-
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Murphy, David,1957-
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Ohio Library and Information Network.
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