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" Methods in Mammary Gland Biology and Breast Cancer Research "
edited by Margot M. Ip, Bonnie B. Asch.
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BL
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| Record Number
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722527
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b542239
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| Main Entry
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edited by Margot M. Ip, Bonnie B. Asch.
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| Title & Author
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Methods in Mammary Gland Biology and Breast Cancer Research\ edited by Margot M. Ip, Bonnie B. Asch.
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| Publication Statement
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Boston, MA: Springer US, 2000
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(xvi, 329 pages)
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| ISBN
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1461542952
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: 9781461542957
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| Contents
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I. In Vivo Model Systems --;1 Mouse Models for Mammary Cancer --;2 Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz anthracene or 1-Methyl-1-Nitrosourea --;3 A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesis --;4 Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease --;5 Implantation and Characterization of Human Breast Carcinomas in SCID Mice --;II. Special Techniques for In Vivo Studies --;6 The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells --;7 Preparing Mammary Gland Whole Mounts from Mice --;8 Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissue --;9 Intramammary Delivery of Hormones, Growth Factors, and Cytokines --;10 Hormonal Stimulation of the Mouse Mammary Gland --;III. In Vitro Model Systems --;11 Collagen Gel Method for the Primary Culture of Mouse Mammary Epithelium --;12 Chemical-Carcinogen-Induced Transformation of Primary Cultures of Mouse Mammary Epithelial Cells Grown inside Collagen Gels --;13 Establishment of Mouse Mammary Cell Lines --;14 Whole Organ Culture of the Mouse Mammary Gland --;15 Working with the Mouse Mammary End Bud --;16 Isolation and Culture of Normal Rat Mammary Epithelial Cells --;17 Characterization of Normal Human Breast Epithelial Cell Subpopulations Isolated by Fluorescence-Activated Cell Sorting and Their Clonogenic Growth In Vitro --;18 Isolation and Culture of Human Breast Cancer Cells from Primary Tumors and Metastases --;IV Molecular Analysis and Gene Transfer Techniques --;19 mRNA In Situ Hybridization in the Mammary Gland --;20 Application of In Situ PCR to Studies of the Mammary Gland --;21 Transfection of Primary Mammary Epithelial Cells by Viral and Nonviral Methods --;22 Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectors --;23 Intraductal Injection into the Mouse Mammary Gland --;24 Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissue --;25 Transplantation and Tissue Recombination Techniques to Study Mammary Gland Biology --;26 Rescue of Mammary Epithelium of Early Lethal Phenotypes by Embryonic Mammary Gland Transplantation as Exemplified with Insulin Receptor Null Mice.
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| Abstract
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This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi- tional money from various federal and state sources, has led to a rapid expansion of research efforts by attracting numerous new investigators into the field.
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Medicine.
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Oncology.
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| LC Classification
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RC280.B8E358 2000
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| Added Entry
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Bonnie B Asch
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Margot M Ip
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