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" Techniques in prion research "
edited by Sylvain Lehmann, Jacques Grassi
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BL
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| Record Number
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733433
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b553231
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| Main Entry
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edited by Sylvain Lehmann, Jacques Grassi
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| Title & Author
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Techniques in prion research\ edited by Sylvain Lehmann, Jacques Grassi
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| Publication Statement
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Basel ; Boston: Birkhäuser, 2004
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| Series Statement
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Methods and tools in biosciences and medicine
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| Page. NO
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x, 219 s. ;
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| ISBN
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3764322241
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: 3764324155
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: 9783764322243
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: 9783764324155
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| Contents
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From the contents: Abbreviations.- Transmissible Spongiform Encephalopathies or Prion Diseases (General Introduction).- Purification of PrPC.- Purification of the Pathological Isoform of Prion Protein (PrPsc or PrP-res) from Transmissible Spongiform Encephalopathy-affected Brain Tissue. Rapid Procedure for Purification and Renaturation of Recombinant PrP Protein.- Animal Models of Transmissible Spongiform Encephalopathies: Experimental Infection, Observation and Tissue Collection.- Cell Culture Models of TSEs. PrPsc Immunohistochemistry.- Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies.- Antibody Production and ELISA, Diagnosis of TSEs, General Considerations.- TSE Strain Typing in Mice.- Biosafety and Decontamination Procedures.- Cell-free Conversion of Prion Proteins.- Cytotoxicity of PrP Peptides.- Cyclic Amplification of Prion Protein Misfolding. Guide to Solutions.- Guide to Protocols.- Index
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| Subject
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Prion diseases -- Laboratory manuals.
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Prions -- isolation purification.
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Prions -- Laboratory manuals.
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| LC Classification
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QR502.E358 2004
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| Added Entry
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Jacques Grassi
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Sylvain Lehmann
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