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" Initial Rate Enzyme Kinetics "
by Herbert J. Fromm.
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BL
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| Record Number
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734684
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b554518
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| Main Entry
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by Herbert J. Fromm.
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| Title & Author
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Initial Rate Enzyme Kinetics\ by Herbert J. Fromm.
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| Publication Statement
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Berlin, Heidelberg: Springer Berlin Heidelberg, 1975
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| Series Statement
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Molecular biology, biochemistry, and biophysics, 22.
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| ISBN
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3642809669
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: 3642809685
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: 9783642809668
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: 9783642809682
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| Contents
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I Nomenclature, Definitions, and Evolution of the Kinetic Mechanism --;A. Nomenclature --;B. Evolution of Initial Rate Kinetics --;References --;II Derivation of Initial Velocity Rate Equations --;A. Definitions and Derivations --;B. The Equilibrium Assumption --;C. Derivation of Complex Steady-State Rate Equations --;D. Derivation of the Rate Equation Using the Rapid Equilibrium Assumption --;E. Derivation of Initial Rate Equations Using a Combination of Equilibrium and Steady-State Assumptions --;F. Derivation of Steady-State Rate Equations by Using the Digital Computer --;References --;III Experimental Protocol and Plotting of Kinetic Data --;A. General Considerations --;B. Analysis of Radioactive Substrates and Determination of Radiopurity --;C. pH Effects --;D. Substrate Concentration --;E. Studies of Forward and Reverse Reactions --;F. Studies of Nucleotide Dependent Enzymic Reactions --;G. The Kinetic Assay --;H. Plotting Methods --;I. Graphical Procedures --;J. The Point of Convergence of Sequential Double Reciprocal Plots as a Criterion of Kinetic Mechanism --;K. Protocol and Data Plotting for Three Substrate Systems --;L. Graphical Methods for Differentiating between Steady-State and Equilibrium Ordered Bi Bi Mechanisms --;References --;IV Use of Competitive Substrate Analogs and Alternative Substrates for Studying Kinetic Mechanisms --;A. Competitive Inhibition --;B. Partial Competitive Inhibition --;C. Noncompetitive Inhibition --;D. Ucompetitive Inhibition --;E. Nonlinear Enzyme Inhibition --;F. The Use of Substrate Analogs for Studying Kinetic Mechanisms --;G. Cleland's Rules for Dead End Inhibition --;H. The Stereochemical Nature of Enzyme and Substrate Interaction --;I. Kinetics of Enzyme Specificity --;J. The Kinetics of Transition State Analogs --;References --;V Product, Substrate, and Alternative Substrate Inhibition --;A. Product Inhibition Experiments --;B. Substrate Inhibition --;C. Alternative Substrate Inhibition --;D. Alternative Product Inhibition --;E. Multisite Ping Pong Mechanisms --;F. Enzymes with Identical Substrate-Product Pairs --;References --;VI Isotope Exchange --;A. Abortive Complex Formation --;B. Derivation of Rate Equations --;C. Substrate Synergism --;D. Calculation of Kinetic Parameters --;E. Experimental Protocol --;F. Isotope-Trapping --;References --;VII Isomerization Mechanisms and the? and Haldane Relationships --;A. The? Relationships --;B. The Haldane Relationships --;C. Isomerization Mechanisms --;References --;VIII The Effect of Temperature and pH on Enzyme Activity --;A. Effect of pH on Enzyme Kinetics --;B. The Effect of Temperature on Enzyme Catalyzed Reactions --;References --;IX Cooperativity and Allostery --;A. Cooperativity --;B. Molecular Models --;C. Kinetic Models --;D. Allostery --;E. Product Effects --;References --;Appendix I Rate Equations, Determinants, and Haldane Expressions for Some Common Kinetic Mechanisms --;Appendix II A Computer Program for Deriving Enzyme Rate Equations --;Appendix III Plotting and Statistical Analysis of Kinetic Data Using the OMNITAB Program.
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| Abstract
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Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community.
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Enzymes -- metabolism.
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Life sciences.
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Medicine.
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| LC Classification
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QP601.B944 1975
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| Added Entry
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Herbert J Fromm
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