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" Medical Biomethods Handbook. "
John M Walker
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BL
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| Record Number
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737455
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b557323
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| Main Entry
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John M Walker
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| Title & Author
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Medical Biomethods Handbook.\ John M Walker
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| Publication Statement
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Totowa, NJ: Humana Press, 2005
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| Series Statement
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Methods in molecular biology.
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(656 pages)
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| ISBN
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1588292886
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: 9781588292889
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| Contents
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Preliminary; Basic Techniques in Molecular Biology; Probe Design, Production, and Applications; Restriction EnzymesTools in Clinical Research; Southern Blotting as a Diagnostic Method; Western Blotting as a Diagnostic Method; Principles and Medical Applications of the Polymerase Chain Reaction; Single-Strand Conformation Polymorphism (SSCP) Analysis; Denaturing Gradient Gel Electrophoresis (DGGE); Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing; Introduction to Capillary Electrophoresis of DNABiomedical Applications; Mapping Techniques. Dideoxyfingerprinting for Mutation DetectionConformation-Sensitive Gel Electrophoresis; Amplification Refractory Mutation System and Molecular Diagnostics; Ligase Chain Reaction; Chemical Cleavage of MismatchTheory and Clinical Applications; The Protein Truncation Test; Linkage, Allele Sharing, and Association; Single-Nucleotide PolymorphismsTechnology and Applications; cDNA Microarrays; Nucleic Acid Sequence-Based Amplification; Oligonucleotide Ligation Assay; Quantitative TaqMan Real-Time PCRDiagnostic and Scientific Applications. Use of Denaturing High-Performance Liquid Chromatography in Molecular MedicineQuantitative PCR; Liquid Chromatography-Mass Spectrometry of Nucleic Acids; Comparative Genomic Hybridization in Clinical and Medical Research; Bioinformatic Tools for Gene and Protein Sequence Analysis; In SituHybridization; Enzyme-Linked Immunosorbent Assay; Protein TherapeuticsMouse, Humanized, and Human Antibodies; Karyotyping; Microsatellite Analysis; Analysis of Chromosomal Translocations; Differential DisplayTheory and Applications; Techniques for Gene Expression Profiling. Capillary Electrophoresis in Clinical AnalysisFlow Cytometry in the Biomedical Arena; Immunocytochemistry; Ribotyping in Clinical Microbiology; Diagnostic Applications of Protein Microarrays; The Human Genome Project; Prenatal Diagnosis of Inborn Errors of Metabolism; Gene TherapyMethods and Application; Back matter.
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| Abstract
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John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cyt.
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| Subject
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Clinical Laboratory Techniques.
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Molecular biology -- Handbooks, manuals, etc.
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Molecular diagnosis -- Handbooks, manuals, etc.
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| LC Classification
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QH506.J646 2005
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John M Walker
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Ralph Rapley
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| Parallel Title
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Methods in Molecular Biology
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