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" Rapid cycle real-time PCR-- methods and applications : "
U. Reischl, C. Wittwer, F. Cockerill, eds.
Document Type
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BL
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Record Number
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748933
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Doc. No
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b568890
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Main Entry
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U. Reischl, C. Wittwer, F. Cockerill, eds.
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Title & Author
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Rapid cycle real-time PCR-- methods and applications : : microbiology and food analysis\ U. Reischl, C. Wittwer, F. Cockerill, eds.
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Publication Statement
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Berlin ; New York : Springer, ©2002.
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Page. NO
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(ix, 258 pages) : illustrations (some color)
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ISBN
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3642483518
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: 9783642483516
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Contents
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From the contents: Use of Real-Time PCR to Monitor Human Herpes-Virus 6 (HHV-6) Reactivation --; Use of Rapid Cycle Real-Time PCR for the Detection of Rabies Virus --; Quantification of Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus from Foods Assumed as Causing Food Poisoning by Using LightCycler --; Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR --; Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products --; Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis --; Rapid and Specific Detection of Coxiella burnetii --; The Quantitation of Chlamydia trachomatis --; Detection of Legionella --; Rapid Detection of Toxigenic Corynebacterium diphteriae --; Duplex LightCycler PCR Assay for the Rapid Detection of Methicilin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation --; Applications and Challenges of Real-Time PCR in the Clinical Microbiological Laboratory.
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Abstract
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Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interested for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
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Subject
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Food -- Microbiology.
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Subject
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Molecular microbiology.
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Subject
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Polymerase chain reaction.
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Added Entry
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C Wittwer
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F Cockerill
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Udo Reischl
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Parallel Title
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Microbiology and food analysis
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