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" Neurocytochemical Methods "
edited by André Calas, Daniel Eugène.
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BL
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| Record Number
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754565
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b574527
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| Main Entry
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edited by André Calas, Daniel Eugène.
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| Title & Author
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Neurocytochemical Methods\ edited by André Calas, Daniel Eugène.
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| Publication Statement
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Berlin, Heidelberg : Springer Berlin Heidelberg, 1991
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| Series Statement
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NATO ASI series., Series H,, Cell biology ;, 58.
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| ISBN
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3642842984
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: 364284300X
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: 9783642842986
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: 9783642843006
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| Contents
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Development and application of antibodies to primary (DA, L-DOPA) and secondary (cGMP) messengers: A technical report --; Hodology --; Transmitter-related retrograde labeling and immunohistochemistry to localize amino acid transmitters in neural pathways and cells --; Ultrastructural identification and quantification of neuroactive amino acids --; Video-microscopic techniques to study axonal transport, neuronal fine structure and cytochemical parameters in live neurons --; Investigating the three-dimensional morphology of fluorescence-labelled neurons using confocal laser scanning microscopy --; Chairman's report --; Detection of Specific Molecules --; Combined cytolabeling methods for investigating chemo-specific neuronal interactions and transmitter co-localization --; Molecular neuroanatomy of synapses, cells and systems in the brain --; Regulated expression of transcription factor and neurotransmitter genes in neural populations: studies using in situ hybridization with radiolabeled oligonucleotide probes --; The use of markers to study the regulation of glial cells in the PNS and CNS --; Chairlady's report --; Quantitative Aspects --; Applications to the study of acetylcholine release of the choline oxidase acetylcholine assay --; Morphometric evaluation of populations of neuronal profiles in the central nervous system --; Positron emission tomography: functional neuroanatomy in vivo --; Microfluorometric imaging of time and space dependent calcium concentration changes in mammalian neurons --; Chairman's report --; Receptors --; Immunocytochemistry of neuropeptide receptors using anti-idiotypic antibodies and antibodies to peptides encoded by complementary RNA --; New approaches for receptor analysis: histochemistry of the GABA/BZ receptors in the rat CNS --; Chairman's report --; Short Papers --; Extracellular dyes induce photodegeneration and photopermeabilization of vertebrate and invertebrate neurons --; A method to study projections of GABAergic neurons using deletion of the GABA immunostaining by metallic ions --; Ultrastructural immunocytochemical localization of choline acetyltransferase in sympathetic ganglia --; A dual immunoperoxidase labelling method to study the ultrastructural relationships between choline acetyltransferase- and neuropeptide Y-containing neurons in the rat striatum --; Characterization of microglia and brain macrophages --; Visualization and quantification of 5-HT1A receptors in the rat brain by radioimmunohistochemistry --; Anti-idiotypic antibodies against opioid receptors: first ultrastructural evidence for a presynaptic inhibition by opioids in the dorsal horn of the rat spinal cord --; Antibodies to complementary peptide for labelling substance P receptors in the dorsal horn of the rat spinal cord for both light and electron microscopy --; Comparative ultrastructural localization of neuropeptide mRNAs using tritiated and biotinylated oligodeoxynucleotide probes --; Use of in situ hybridization and immunohistochemistry to study the effects of intranigral injections of colchicine on enkephalin- and substance P-containing neurons of the limbic forebrain --; Chairman's report --; Author Index.
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| Abstract
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The book is aimed at providing researchers, postgraduate students, neurologists, and biological laboratory staff with accurate and up-to-date descriptions of most recent and powerful neurocytochemical methods: localization and co-localization of neurotransmitters; study of their cellular and sub-cellular interactions; histological and ultrastructural detection of receptors; quantification of mediators;hodology and 3-D reconstruction of neurons; cytofunctional approach of axonal transport, of genic expression, and of physiological effects of neurotransmitters (PET, voltage- or ion-sensitive dyes). The possibilities limits, technical difficulties, and applications of these techniques and of their possible combinations on the same tissue are emphasized.
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Human physiology.
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Medicine.
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Neurosciences.
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| LC Classification
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QP356.3E358 1991
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| Added Entry
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André Calas
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Daniel Eugène
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| Parallel Title
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Proceedings of the NATO Advanced Research Workshop on Recent Advances in Neurocytochemical Methods held in Seillac, France, September 26-28, 1990
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