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" Nonradioactive Labeling and Detection of Biomolecules "
edited by Christoph Kessler.
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BL
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| Record Number
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758752
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b578718
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| Main Entry
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edited by Christoph Kessler.
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| Title & Author
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Nonradioactive Labeling and Detection of Biomolecules\ edited by Christoph Kessler.
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| Publication Statement
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Berlin, Heidelberg : Springer Berlin Heidelberg, 1992
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| Series Statement
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Springer laboratory.
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| Page. NO
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(xxiii, 436 pages 58 illustrations)
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| ISBN
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3662001446
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: 9783662001448
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| Contents
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1 General Aspects of Nonradioactive Labeling and Detection --; 1.1 Introduction --; 1.2 The Concept of Nonradioactive Bioanalytics. --; 1.3 Direct and Indirect Labeling and Detection Systems --; 1.4 Labeling Methods --; 1.5 Detection Methods --; 1.6 Guide of the Use of Information in this Book --; I Standard Nonradioactive Labeling and Detection Systems --; 2 Overview of Nonradioactive Labeling Systems --; 3 The Digoxigenin: Anti-Digoxigenin (DIG) System --; 4 The Biotin System --; 5 In Vivo Labeling of DNA Probes with 5-BrdU --; 6 The Sulfone System --; 7 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes --; 8 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection --; 9 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays --; 10 The SNAP System --; II Specialized Nonradioactive Detection Systems --; 11 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems --; 12 Colorimetric Systems --; 13 Luminescence Systems --; 14 Fluorescence Systems --; III Enhanced Systems --; 15 Overview of Amplification Systems --; 16 Enhanced Signal Generation by Target Amplification --; 17 Signal Amplification System: Substrate Cascade --; IV Application Formats --; 18 Overview of Application Formats --; 19 Blot Formats: Nucleic Acids --; 20 Blot Formats: Proteins and Glycoproteins --; 21 In Situ Formats --; 22 Quantitative Formats --; Appendix I Suppliers of Reagents --; Appendix II Suppliers of Equipment.
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| Abstract
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All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
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| Subject
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Biochemistry.
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Cytology.
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Life sciences.
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| LC Classification
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QP519.9.A37E358 1992
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| Added Entry
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Christoph Kessler
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