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" A laboratory guide to genomic sequencing : "
Hans-Peter Saluz, Jean-Pierre Jost.
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BL
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| Record Number
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767999
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b587985
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| Main Entry
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Hans-Peter Saluz, Jean-Pierre Jost.
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| Title & Author
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A laboratory guide to genomic sequencing : : the direct sequencing of native uncloned DNA\ Hans-Peter Saluz, Jean-Pierre Jost.
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| Publication Statement
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Basel ; Boston : Birkhauser, 1987
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| Series Statement
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BioMethods, 1
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163 p. ; 24 cm.
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| ISBN
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3764319259
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: 9783764319250
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| Contents
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I. Introduction.- A Safety Considerations.- B Introduction.- C The Principle of Genomic Sequencing.- II Theoretical Background.- A Basic Theoiy of Genomic Sequencing.- B Flow Diagram.- III Experimental.- 1 Isolation of Genomic DNA.- 2 Restriction Digest of Genomic DNA.- 3 Chemical Sequencing Reactions on Restricted DNA.- 4 Separation of Reaction Products on a Sequencing Gel.- 5 Electrotransfer to Nylon Membranes.- 6 Immobilization of DNA on a Nylon Membrane.- 7 Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes.- 8 Processing of the Hybridized Filters.- 9 Autoradiography and Photography.- 10 Cloning of DNA Probe in M13.- 11 Large-Scale Preparation of Cloned DNA in M13.- 12 Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes.- 13 Purification of Labeled Single-Stranded Probes.- IV Trouble-Shooting Guide and Examples.- V Appendix.- A Suppliers of Special Items and Constructions of Commercially Unavailable Equipment.- B Determination of DNA Concentration.- VI Bibliography.
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| LC Classification
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QP625.N89H367 1987
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| Added Entry
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Hans Peter Saluz
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Jean Pierre Jost
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