رکورد قبلیرکورد بعدی

" Visualisation of ribosomal subunits interaction reveals 80s ribosomes in the nucleus of Drosophila cells "


Document Type : Latin Dissertation
Record Number : 806977
Doc. No : TLets636864
Main Entry : Abdullahi, Akilu Sada
Title & Author : Visualisation of ribosomal subunits interaction reveals 80s ribosomes in the nucleus of Drosophila cells\ Abdullahi, Akilu Sada
College : University of Birmingham
Date : 2015
student score : 2015
Degree : Thesis (Ph.D.)
Abstract : In eukaryotes, transcription and RNA processing events are spatially separated from translation by the nuclear envelope. Although ribosomal subunits are synthesised and assembled in the nucleus, it is believed that they are kept inactive whilst in the nucleus. Yet, there were observations from this and other laboratories that suggest translation can occur in the nucleus. To further investigate whether translating ribosomes exist in the nucleus, I employed a bimolecular fluorescence complementation (BiFC) 80S reporter based technique previously developed in our laboratory that detects 80S assembly in \(Drosophila\) cells. The initial characterization indicated that the assay reports ribosomal subunit interaction, but other explanations could not be excluded. My first aim was to assess whether this technique is genuinely reporting translation-dependent subunits association. My results were consistent with the assay reporting 80S ribosomes formed as a consequence of translation. Following up from this, I developed a similar technique with Venus, which resulted in a more sensitive 80S reporter. While the 80S signal was more apparent in the cytoplasm, in both cell culture and fly tissue cells, a fraction of the cells showed a signal in the nucleus, particularly concentrated in the nucleolus. This signal was enhanced by translation elongation inhibitors in both cytoplasm and nucleus indicating that the detected 80S are engaged in translation. Notably, the nucleolar signal was prevented by RNA Pol II inhibition, suggesting that the 80S might be associated with mRNA in the nucleolus.
Subject : QR Microbiology
Added Entry : University of Birmingham
کپی لینک

پیشنهاد خرید
پیوستها
عنوان :
نام فایل :
نوع عام محتوا :
نوع ماده :
فرمت :
سایز :
عرض :
طول :
ets636864_11835.pdf
ets636864.pdf
پایان نامه لاتین
متن
application/pdf
25.05 MB
85
85
نظرسنجی
نظرسنجی منابع دیجیتال

1 - آیا از کیفیت منابع دیجیتال راضی هستید؟