Fluorigenic enzyme assays using long-wavelength substrates

منو

" Fluorigenic enzyme assays using long-wavelength substrates "
Sarpara, Ghulam Hussain

Document Type	:	Latin Dissertation
Record Number	:	829214
Doc. No	:	TLets484291
Main Entry	:	Sarpara, Ghulam Hussain
Title & Author	:	Fluorigenic enzyme assays using long-wavelength substrates\ Sarpara, Ghulam Hussain
College	:	Loughborough University
Date	:	2000
student score	:	2000
Degree	:	Thesis (Ph.D.)
Abstract	:	Investigations into the use of long-wavelength fluorigenic substrates, which are non- or weakly fluorescent but are converted by an appropriate enzyme to a highly fluorescent product, have been carried out in order to determine several different enzymes and their inhibitors. Certain nonfluorescent compounds based on long-wavelength xanthenes and oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate and naphthofluorescein mono-phosphate has been demonstrated to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis product of these substrates can be used for trace analyses using diode laser based fluorescence detection.
Subject	:	Organic chemistry
Added Entry	:	Loughborough University