| Document Type
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BL
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| Record Number
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839748
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| Title & Author
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Bioprocessing technology for production of biopharmaceuticals and bioproducts /\ edited by Claire Komives, Weichang Zhou.
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| Edition Statement
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First edition.
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| Publication Statement
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Hoboken, NJ :: John Wiley & Sons, Inc.,, 2019.
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| Series Statement
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Wiley series in biotechnology and bioengineering
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| Page. NO
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1 online resource (xiii, 273)
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| ISBN
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1119378281
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: 1119378303
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: 1119378346
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: 9781119378280
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: 9781119378303
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: 9781119378341
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9781118361986
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Cover; Title Page; Copyright; Contents; List of Contributors; Part I Case Study; Chapter 1 Bacillus and the Story of Protein Secretion and Production; 1.1 Bacillus as a Production Host: Introduction and Historical Account; 1.2 The Building of a Production Strain: Genetic Tools for B. subtilis Manipulation; 1.2.1 Promoters; 1.2.2 Vectors for Building a Production Strain; 1.2.3 B. subtilis Competent Cell Transformation; 1.2.4 Protoplasts-Mediated Manipulations; 1.2.5 Genetics by Electroporation; 1.3 B. subtilis Secretion System and Heterologous Protein Production
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1.3.1 Bacillus Fermentation and Recovery of Industrial Enzyme1.3.2 Fermentation Stoichiometry; 1.3.3 Fermentor Kinetics and Outputs; 1.3.4 Downstream Processing; 1.4 Summary; References; Chapter 2 New Expression Systems for GPCRs; 2.1 Introduction; 2.2 Recombinant GPCR Production -- Traditional Approaches for Achieving High-Level Production; 2.3 Engineered Expression Systems for GPCR Production; 2.3.1 Bacteria; 2.3.2 Yeasts; 2.3.3 Insect Cells; 2.3.4 Mammalian Cells; 2.3.5 Transgenic Animals; 2.3.6 Cell-Free Systems; 2.4 Conclusion; References; Chapter 3 Glycosylation; 3.1 Introduction
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3.2 Types of Glycosylation3.2.1 N-linked Glycans; 3.2.2 O-linked Glycans; 3.3 Factors Affecting Glycosylation; 3.3.1 Nutrient Depletion; 3.3.2 Fed-batch Cultures and Supplements; 3.3.3 Specific Culture Supplements; 3.3.4 Ammonia; 3.3.5 pH; 3.3.6 Oxygen; 3.3.7 Host Cell Systems; 3.3.8 Other Factors; 3.4 Modification of Glycosylation; 3.4.1 siRNA and Gene Knockout/Knockin; 3.4.2 Glycoprotein Processing Inhibitors and In Vitro Modification of Glycans; 3.5 Glycosylation Analysis; 3.5.1 Release of Glycans from Glycoproteins; 3.5.2 Derivatization of Glycans; 3.6 Methods of Analysis
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3.6.1 Lectin Arrays3.6.2 Liquid Chromatography; 3.6.2.1 HILIC Analysis; 3.6.2.2 Reversed Phase (RP) and Porous Graphitic Carbon (PGC) Chromatography; 3.6.2.3 Weak Anion Exchange (WAX) HPLC Analysis; 3.6.2.4 High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD); 3.6.3 Capillary Electrophoresis (CE); 3.6.4 Fluorophore-assisted Carbohydrate Electrophoresis (FACE) and CGE-LIF; 3.6.5 Mass Spectrometry (MS); 3.6.5.1 Ionization; 3.6.5.2 Derivatization Techniques Used for MS Analysis of Glycans; 3.6.5.3 Fragmentation of Carbohydrates; 3.7 Conclusion; References
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Part II BioreactorsChapter 4 Bioreactors for Stem Cell and Mammalian Cell Cultivation; 4.1 Overview of (Mammalian and Stem) Cell Culture Engineering; 4.1.1 Cell Products for Therapeutics; 4.1.2 Cell as a Product: Stem Cells; 4.2 Bioprocess Characterization; 4.2.1 Cell Cultivation Methods; 4.2.2 Cell Metabolism; 4.2.3 Culture Medium Design; 4.2.4 Culture Parameters; 4.2.5 Culture Modes; 4.3 Cell Culture Systems; 4.3.1 Static Culture Systems; 4.3.2 Roller Bottles; 4.3.3 Spinner Flask; 4.3.4 Airlift Bioreactor; 4.3.5 Fixed/Fluidized-Bed Bioreactor; 4.3.6 Wave Bioreactor
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| Abstract
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"Written for industrial and academic researchers and development scientists in the life sciences industry, Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts is a guide to the tools, approaches, and useful developments in bioprocessing. This important guide: Summarizes state-of-the-art bioprocessing methods and reviews applications in life science industries Includes illustrative case studies that review six milestone bio-products Discusses a wide selection of host strain types and disruptive bioprocess technologies"--Provided by publisher.
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| Subject
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Biotechnological process monitoring.
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Pharmaceutical biotechnology industry.
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Biotechnological process monitoring.
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MEDICAL-- Pharmacology.
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Pharmaceutical biotechnology industry.
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Biological Products-- chemistry.
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Bioreactors.
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Drug Compounding.
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| Subject
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Technology, Pharmaceutical.
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| Dewey Classification
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615.1/9
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| LC Classification
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RM301.25.B56 2019
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| NLM classification
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QV 241.B615 2019
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| Added Entry
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Komives, Claire
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Zhou, Weichang,1963-
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