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BL
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| Record Number
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846489
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| Main Entry
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Sengar, Rakesh S.
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| Title & Author
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Advances in Molecular Techniques /\ Rakesh S. Sengar, Amit Kumar, Reshu Chaudhary, Ashu Singh.
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| Edition Statement
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First edition.
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| Publication Statement
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Boca Raton, FL :: CRC Press,, 2018.
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| Page. NO
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1 online resource :: text file, PDF
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| ISBN
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1351021206
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: 1351021214
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: 1351021222
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: 9781351021203
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: 9781351021210
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: 9781351021227
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081537075X
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9780815370758
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation.
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Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions.
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Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals.
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MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required.
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Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required.
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| Abstract
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"Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists. This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering."--Provided by publisher.
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| Subject
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Biotechnology.
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Genetics.
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Molecular biology.
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Biotechnology.
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Genetics.
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Molecular biology.
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SCIENCE-- Life Sciences-- Biochemistry.
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Genetic Techniques.
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| Subject
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Computational Biology-- methods.
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| Dewey Classification
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SCBS0755
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SCBS0770
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SCBS09
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WB042
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WB057
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WB061
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WB071
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WB075
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| LC Classification
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QH442
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| NLM classification
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2018 G-016
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QU 550
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| Added Entry
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Chaudhary, Reshu
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Kumar, Amit
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Singh, Ashu
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