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BL
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| Record Number
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851668
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| Title & Author
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Electrophoretic separation of proteins : : methods and protocols /\ edited by Biji T. Kurien and R. Hal Scofield.
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| Publication Statement
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New York :: Humana Press :: Springer,, [2018]
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, ©2019
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| Series Statement
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Methods in molecular biology ;; v. 1855
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| Page. NO
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1 online resource (xv, 523 pages) :: illustrations (some color)
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| ISBN
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1493987933
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: 9781493987931
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9781493987924
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Intro; Preface; Contents; Contributors; Chapter 1: How It All Began: A Personal History of Gel Electrophoresis; 1 Introduction; References; Chapter 2: Introduction to Protein Electrophoresis; 1 Introduction; 2 Electrophoresis on a Solid Support; 2.1 Paper Electrophoresis; 2.2 Gel Electrophoresis; 2.2.1 Polyacrylamide Gel Electrophoresis (PAGE); 2.2.2 Buffers; 2.2.3 Two-Dimensional Gel Electrophoresis; 2.2.4 Capillary Electrophoresis; References; Chapter 3: Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid As ... ; 1 Introduction
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1.1 Detecting Proteins with Absorbance at 280 nm1.2 Detecting Proteins with the Lowry Method; 1.3 Detecting Proteins with the Smith Bicinchoninic Acid Method; 1.4 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 2 Materials; 2.1 Detecting Proteins by Measuring Absorbance at 280 nm or Using a Standard Curve of Absorbance Against Protein Concentration ... ; 2.2 Detecting Proteins with the Lowry Method; 2.3 Detecting Proteins with the Smith Bicinchoninic Acid Method; 2.4 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 3 Methods
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1.11 Concentrating Proteins with Antibodies in Immunoprecipitation Assays1.12 Concentrating Proteins Using Thermal Denaturation; 1.13 Concentrating Protein Solutions for Proteomic and Peptidomic Studies; 2 Materials; 2.1 Concentrating Proteins by Precipitation with Ammonium Sulfate and Polyethylene Glycol; 2.2 Concentrating Proteins by Precipitation with Organic Solvents and Trichloroacetic Acid (TCA); 2.3 Concentrating Proteins for SDS-PAGE with SDS/KCl Precipitation; 2.4 Concentrating Proteins with Three-Phase Partitioning; 2.5 Concentrating Protein by the Absorption of Solvent
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1.2 Concentrating Proteins by Precipitation with Organic Solvents and Trichloroacetic Acid (TCA)1.3 Concentrating Proteins for SDS-PAGE with SDS/KCl Precipitation; 1.4 Concentrating Proteins with Three-Phase Partitioning; 1.5 Concentrating Protein by the Absorption of Solvent; 1.6 Centrifugation to Concentrate Proteins; 1.7 Concentrating Proteins with Increased Pressure; 1.8 Concentrating Protein Solutions Using a Vacuum; 1.9 Concentrating Protein Solutions Using a Vacuum and Freezing: Freeze-Drying (Lyophilization); 1.10 Concentrating Proteins Using Affinity Chromatography
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3.1 Detecting Proteins by Measuring Absorbance at 280 nm3.2 Detecting Proteins Using a Standard Curve of Protein Against Absorbance at 280 nm; 3.3 Detecting Proteins with the Lowry Method; 3.4 Detecting Proteins with the Smith Bicinchoninic Acid Method; 3.5 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 4 Notes; References; Chapter 4: Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis ... ; 1 Introduction; 1.1 Concentrating Proteins by Precipitation with Salts and Polyethylene Glycol
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| Subject
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Electrophoresis, Laboratory manuals.
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Proteins-- Separation, Laboratory manuals.
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Chemistry Techniques, Analytical.
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Electrochemical Techniques.
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| Subject
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Electrophoresis-- methods.
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Electrophoresis, Agar Gel.
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Immunoelectrophoresis.
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Laboratory Manuals
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Proteins.
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Electrophoresis.
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Proteins-- Separation.
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Proteins.
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Science-- Life Sciences-- Biochemistry.
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| Dewey Classification
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541/.372
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| LC Classification
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QP519.9.E434
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| Added Entry
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Kurien, Biji T.
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Scofield, R. Hal
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