| Document Type
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BL
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| Record Number
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851677
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| Title & Author
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Protein misfolding diseases : : methods and protocols /\ editor Cláudio M. Gomes.
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| Publication Statement
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New York, NY :: Humana Press :: Springer,, ©2018.
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| Series Statement
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Methods in Molecular Biology,; volume 1873
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| Page. NO
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1 online resource (xiii, 338 pages) :: illustrations (some color)
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| ISBN
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1493988204
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: 9781493988204
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1493988190
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9781493988198
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Intro; Preface; Contents; Contributors; Part I: Protein Biophysics Assays; Chapter 1: Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation; 1 Introduction; 1.1 Protein Misfolding and Aggregation in Disease; 1.2 Circular Dichroism; 1.3 Fourier-Transform Infrared Spectroscopy; 1.4 Fluorescence Spectroscopy; 1.5 Dynamic Light Scattering; 2 Materials; 2.1 SOD1 Expression and Purification; 2.2 Demetallation of SOD1; 2.3 CD; 2.4 ATR-FTIR; 2.5 1,8-ANS Fluorescence; 2.6 ThT Fluorescence Kinetics; 2.7 DLS; 3 Methods; 3.1 SOD1 Expression and Purification
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3.2.1 Stock Solutions3.2.2 Prepreparation; 3.2.3 Multilamellar Dispersion (MLD); 3.2.4 Small Unilamellar Vesicles (SUVs), Size Range 1000 nm; 3.3 Fluorescence Measurements; 3.3.1 Förster Resonance Energy Transfer (FRET); 3.3.2 Fluorescence Anisotropy; 3.3.3 Fluorescence Quenching; 3.3.4 Vesicle Leakage Assay; 3.4 Calorimetric Measurements; 3.4.1 Differential Scanning Calorimetry (DSC); 3.4.2 Isothermal Titration Calorimetry (ITC); 3.5 Electron Spin Resonance (ESR); 3.6 Microscopy; 4 Notes
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| Subject
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Protein folding.
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| Subject
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Proteostasis Deficiencies.
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| Subject
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Protein folding.
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| Dewey Classification
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572/.633
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| LC Classification
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QP551
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| Added Entry
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Gomes, Cláudio M.,1970-
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