| Document Type
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BL
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| Record Number
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851758
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| Title & Author
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Alpha-synuclein : : methods and protocols /\ edited by Tim Bartels.
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| Publication Statement
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New York, NY :: Humana Press :: Springer,, [2019]
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, ©2019
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| Series Statement
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Methods in molecular biology,; 1948
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| Page. NO
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1 online resource (xii, 289 pages) :: illustrations (some color)
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| ISBN
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1493991248
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: 9781493991242
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149399123X
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9781493991235
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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2.3 Tissue Sectioning2.4 Deparaffinization; 2.5 Antigen Retrieval and Staining; 3 Methods; 3.1 Tissue Fixation; 3.2 Tissue Paraffinization and Sectioning; 3.3 Deparaffinization, Antigen Retrieval, and Staining; 4 Notes; References; Chapter 3: Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons; 1 Introduction; 2 Materials; 2.1 Neuronal Dissection; 2.2 Neuronal Transfection; 2.3 vGLUT-pHluorin Imaging; 2.4 Data Analysis; 3 Methods; 3.1 Pasteur Pipet Siliconization; 3.2 Coverslip Treatment; 3.3 Coverslip Coating; 3.4 Hippocampal Dissection; 3.5 Neuron Disaggregation
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4 NotesReferences; Chapter 5: Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain Using Preformed Fibrils; 1 Introduction; 2 Materials; 2.1 Sonication of aSyn PFFs; 2.2 Stereotaxic Injection into Mouse Brain; 2.3 Mouse Perfusion and Confirmation of Pathology by Immunohistochemistry; 3 Methods; 3.1 Sonication of aSyn PFFs; 3.2 Stereotaxic Injection into Mouse Brain; 3.3 Coordinates for Different CNS Sites; 3.4 Mouse Perfusion and Confirmation of Pathology by Immunohistochemistry; 3.5 Decontamination of Instruments and Work Surfaces; 4 Notes; References
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Chapter 6: Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection1 Introduction; 2 Materials; 3 Methods; 3.1 Preparation of Biospecimen; 3.2 SULFO-TAG Labeling of Detection Antibodies; 3.3 Coating of Assay Plates; 3.4 Washing; 3.5 Blocking; 3.6 Preparation of Standard Curves; 3.7 Sample Dilution; 3.8 Application of Standards and Samples to the Assay Plate; 3.9 Application of Detection Antibody; 3.10 Measurement; 3.11 Data Analysis; 4 Notes; References
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| Subject
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Alpha-synuclein, Laboratory manuals.
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| Subject
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Alpha-synuclein.
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| Subject
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alpha-Synuclein.
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| Dewey Classification
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572/.696
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| LC Classification
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QP552.A4397
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| Added Entry
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Bartels, Tim
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