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" X-ray free electron lasers : "
Sébastien Boutet, Petra Fromme, Mark S. Hunter, editors.
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BL
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| Record Number
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859217
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| Title & Author
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X-ray free electron lasers : : a revolution in structural biology /\ Sébastien Boutet, Petra Fromme, Mark S. Hunter, editors.
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| Publication Statement
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Cham, Switzerland :: Springer,, [2018]
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1 online resource
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| ISBN
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3030005518
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: 9783030005511
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303000550X
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9783030005504
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| Contents
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Intro; Preface; Contents; Contributors; 1 X-Ray Free Electron Lasers and Their Applications; 1.1 X-Rays and Their Applications; 1.1.1 X-Ray Methods; 1.1.1.1 Scattering; 1.1.1.2 Spectroscopy; 1.1.2 The Evolution of X-Ray Sources; 1.2 X-Ray FELs as User Facilities; 1.2.1 The Physics of Free Electron Lasers; 1.3 The Scientific Applications of X-Ray FELs; 1.3.1 Using the Time Resolution; 1.3.2 Using the High Peak Intensity; 1.3.2.1 Diffraction-Before-Destruction; 1.3.2.2 Using FELs to Create New States of Matter; 1.3.2.3 Nonlinear X-Ray Physics; 1.3.3 Using the Coherence; 1.4 Summary; References
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2 Serial Femtosecond Crystallography (SFX): An Overview2.1 Background; 2.2 The Need for SFX; 2.2.1 Sample Preparation and Characterization for SFX; 2.2.2 Sample Introduction for SFX; 2.2.3 Data Analysis for SFX; 2.3 Early Experiments of Serial Femtosecond Crystallography (SFX); 2.3.1 The "Birth" of SFX; 2.3.2 SFX at High Resolution; 2.3.3 SFX of G-Protein Coupled Receptors; 2.3.4 Phasing of SFX Data; 2.3.5 Time-Resolved SFX; 2.3.6 Imperfect Crystals and the Push Toward Crystal-Free Imaging; 2.3.7 Towards Single Particle X-ray Diffraction
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2.3.8 Structure Determination Based on fs Small and Wide Angle Solution X-ray Scattering2.4 Pushing SFX Forward; 2.4.1 Serial Operations at Beamlines for SFX at Current X-ray FELs; 2.4.2 New Experimental Endstations for X-ray FEL Experiments; 2.4.3 Higher Repetition Rate X-ray FELs; 2.5 Outlook; References; 3 Small Is Beautiful: Growth and Detection of Nanocrystals; 3.1 Introduction; 3.2 Nanocrystallogenesis; 3.2.1 Thermodynamics of Solubility and Nucleation; 3.2.2 Methods; 3.2.2.1 Adaptation of Existing Conditions; 3.2.2.2 Free Interface Diffusion; 3.2.2.3 Batch Nanocrystallization
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3.2.2.4 Other Methods and Sample Delivery Considerations3.2.3 Stability and Storage; 3.3 Considerations and Characterization for SFX Optimization; 3.3.1 Characteristics: What Is Optimal?; 3.3.1.1 Data Analysis Considerations; 3.3.1.2 Practicality: Sample and Hardware; 3.3.1.3 Control of Homogeneity Through Post Growth Methods; 3.3.2 Characterization; 3.3.2.1 Optical Detection: Visualizing Your Crystals; 3.3.2.2 Light Scattering Techniques for Size Determination; 3.3.2.3 Transmission Electron Microscopy; 3.3.2.4 Powder Diffraction; 3.4 Recap; References
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4 The Lipid Cubic Phase as a Medium for the Growth of Membrane Protein Microcrystals4.1 Introduction; 4.2 Procedure for the Preparation and Characterization of Microcrystals for LCP-SFX; 4.2.1 Membrane Protein Reconstitution in LCP (Modified from Ref. [28]); 4.2.2 Sample Consolidation and Titration With 7.9 MAG; 4.2.3 Microcrystal Characterization; 4.2.4 Adjusting Crystal Density; 4.2.5 Loading the Sample in an LCP Injector for LCP-SFX Data Collection; 4.3 Results; 4.4 Conclusion; References; 5 Sample Delivery Techniques for Serial Crystallography; 5.1 Overview
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| Abstract
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The timely volume describes recent discoveries and method developments that have revolutionized Structural Biology with the advent of X-ray Free Electron Lasers. It provides, for the first time, a comprehensive examination of this cutting-edge technology. It discusses of-the-moment topics such as growth and detection of nanocrystals, Sample Delivery Techniques for serial femtosecond crystallography, data collection methods at XFELs, and more. This book aims to provide the readers with an overview of the new methods that have been recently developed as well as a prospective on new methods under development. It highlights the most important and novel Structural Discoveries made recently with XFELS, contextualized with a big-picture discussion of future developments.
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| Subject
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Free electron lasers.
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Free electron lasers.
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| Dewey Classification
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621.36/6
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| LC Classification
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TA1693.X73 2018
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| Added Entry
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Boutet, Sébastien
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Fromme, Petra
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Hunter, Mark S.
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