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" Transgenic ascidians / "
Yasunori Sasakura, editor.
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BL
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| Record Number
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889138
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| Title & Author
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Transgenic ascidians /\ Yasunori Sasakura, editor.
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| Publication Statement
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Singapore, Singapore :: Springer,, [2018]
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| Series Statement
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Advances in experimental medicine and biology,; volume 1029
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1 online resource
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| ISBN
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9789811075452
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: 981107545X
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9789811075445
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9811075441
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Introduction / Yasunori Sasakura -- Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou -- Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall -- Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano -- Electroporation in ascidians: history, theory and protocols / Robert W. Zeller -- The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe -- Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian Cañestro -- investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio -- Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen -- Germline transgenesis in Ciona / Yasunori Sasakura -- The enhancer trap in Ciona / Yasunori Sasakura -- TALEN-based knockout system / Keita Yoshida, Nicholas Treen -- CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi -- Transgenic Techniques for Investigating Cell Biology During Development / Christina D. Cota -- Cellular processes of notochord formation / William C. Smith -- Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino.
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| Abstract
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This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
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Biology-- Technique.
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Cytology.
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Developmental biology.
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Evolution (Biology)
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Sea squirts.
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Transgenic organisms.
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Biology-- Technique.
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Cytology.
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Developmental biology.
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Evolution (Biology)
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SCIENCE-- Life Sciences-- Anatomy Physiology.
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Sea squirts.
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Transgenic organisms.
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Genetic Engineering-- methods.
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Urochordata-- genetics.
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Animals, Genetically Modified.
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Transgenes.
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| Dewey Classification
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571.8
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| LC Classification
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QH442.6
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| NLM classification
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QH 442.6
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| Added Entry
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Sasakura, Yasunori
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