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" Cytogenetic map of common bean (Phaseolus vulgaris L.) "
Fonsêca, ArturFerreira, JoanaSantos, Tiago RibeiroMosiolek, MagdalenaBellucci, ElisaKami, JamesGepts, PaulGeffroy, ValérieSchweizer, DieterSantos, Karla G.Pedrosa-Harand, Andrea
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AL
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| Record Number
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900172
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LA8g71d7td
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| Title & Author
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Cytogenetic map of common bean (Phaseolus vulgaris L.) [Article]\ Fonsêca, ArturFerreira, JoanaSantos, Tiago RibeiroMosiolek, MagdalenaBellucci, ElisaKami, JamesGepts, PaulGeffroy, ValérieSchweizer, DieterSantos, Karla G.Pedrosa-Harand, Andrea
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2010
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UC Davis
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| Abstract
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A cytogenetic map of common bean was built by in situ hybridization of 35 bacterial artificial chromosomes (BACs) selected with markers mapping to eight linkage groups, plus two plasmids for 5S and 45S ribosomal DNA and one bacteriophage. Together with three previously mapped chromosomes (chromosomes 3, 4, and 7), 43 anchoring points between the genetic map and the cytogenetic map of the species are now available. Furthermore, a subset of four BAC clones was proposed to identify the 11 chromosome pairs of the standard cultivar BAT93. Three of these BACs labelled more than a single chromosome pair, indicating the presence of repetitive DNA in their inserts. A repetitive distribution pattern was observed for most of the BACs; for 38% of them, highly repetitive pericentromeric or subtelomeric signals were observed. These distribution patterns corresponded to pericentromeric and subtelomeric heterochromatin blocks observed with other staining methods. Altogether, the results indicate that around half of the common bean genome is heterochromatic and that genes and repetitive sequences are intermingled in the euchromatin and heterochromatin of the species.
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