Combined aptamer and transcriptome sequencing of single cells.

منو

" Combined aptamer and transcriptome sequencing of single cells. "
Delley, Cyrille LLiu, LeqianSarhan, Maen FAbate, Adam R

Document Type	:	AL
Record Number	:	901302
Doc. No	:	LA2h34n1jx
Title & Author	:	Combined aptamer and transcriptome sequencing of single cells. [Article]\ Delley, Cyrille LLiu, LeqianSarhan, Maen FAbate, Adam R
Date	:	2018
Title of Periodical	:	UCSF
Abstract	:	The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.