|
" Functional assessment of hematopoietic niche cells derived from human embryonic stem cells. "
Ferrell, Patrick IHexum, Melinda KKopher, Ross ALepley, Michael AGussiaas, AmandaKaufman, Dan Set al.
| Document Type
|
:
|
AL
|
| Record Number
|
:
|
901319
|
| Doc. No
|
:
|
LA4s74z92t
|
| Title & Author
|
:
|
Functional assessment of hematopoietic niche cells derived from human embryonic stem cells. [Article]\ Ferrell, Patrick IHexum, Melinda KKopher, Ross ALepley, Michael AGussiaas, AmandaKaufman, Dan Set al.
|
| Date
|
:
|
2014
|
| Title of Periodical
|
:
|
UC San Diego
|
| Abstract
|
:
|
To evaluate hematopoietic niche cell populations isolated from human embryonic stem cells (hESCs), we tested the ability of hESC-derived stromal lines to support CD34(+) umbilical cord blood (UCB)- and hESC-derived CD34(+)45(+) cells in long-term culture initiating cell (LTC-IC) assays. Specifically, these hematopoietic populations were cocultured with hESC-derived mesenchymal stromal cells (hESC-MSCs) and hESC-derived endothelial cells (hESC-ECs), and then assessed for their LTC-IC potential in comparison to coculture with bone marrow (BM)-derived MSCs and the mouse stromal line M2-10B4. We found that the hESC-derived stromal lines supported LTC-ICs from UCB similar to M2-10B4 cells and better than BM-MSCs. However, none of the stromal populations supported LTC-IC from hESC-derived CD34(+)45(+) cells. Engraftment data using the output from LTC-IC assays showed long-term repopulation (12 weeks) of NSG mice to correlate with LTC-IC support on a given stromal layer. Therefore, hESC-derived stromal lines can be used to efficiently evaluate putative hematopoietic stem/progenitor cells derived from hESCs or other cell sources.
|
| |