A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resist...

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" A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus. "
Valderrama, J Andrés; Kulkarni, Surashree S; Nizet, Victor; Bier, Ethan

Document Type	:	AL
Record Number	:	908118
Doc. No	:	LA5v13x4b4
Title & Author	:	A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus. [Article]\ Valderrama, J Andrés; Kulkarni, Surashree S; Nizet, Victor; Bier, Ethan
Date	:	2019
Title of Periodical	:	UC San Diego
Abstract	:	Gene-drive systems in diploid organisms bias the inheritance of one allele over another. CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the gRNA directs Cas9-mediated cleavage. In the presence of Cas9, the gRNA cassette and any linked cargo sequences are copied via homology-directed repair (HDR) onto the homologous chromosome. Here, we develop an analogous CRISPR-based gene-drive system for the bacterium Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This "pro-active" genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with ~ 100-fold greater efficiency than control CRISPR-based methods, suggesting an amplifying positive feedback loop due to increasing gRNA dosage. Pro-AG can likewise effectively edit large plasmids or single-copy genomic targets or introduce functional genes, foreshadowing potential applications to biotechnology or biomedicine.