|
" pH- and thermo-sensitive MTX-loaded magnetic nanocomposites: "
Farshbaf, Masoud; Salehi, Roya; Annabi, Nasim; Khalilov, Rovshan; Akbarzadeh, Abolfazl; Davaran, Soodabeh
| Document Type
|
:
|
AL
|
| Record Number
|
:
|
910066
|
| Doc. No
|
:
|
LA4t38s3t6
|
| Title & Author
|
:
|
pH- and thermo-sensitive MTX-loaded magnetic nanocomposites:. synthesis, characterization, and in vitro studies on A549 lung cancer cell and MR imaging. [Article]\ Farshbaf, Masoud; Salehi, Roya; Annabi, Nasim; Khalilov, Rovshan; Akbarzadeh, Abolfazl; Davaran, Soodabeh
|
| Date
|
:
|
2018
|
| Title of Periodical
|
:
|
UCLA
|
| Abstract
|
:
|
In the current study, we proposed a facile method for fabrication of multifunctional pH- and thermo-sensitive magnetic nanocomposites (MNCs) as a theranostic agent for using in targeted drug delivery and magnetic resonance imaging (MRI). To this end, we decorated Fe3O4 magnetic nanoparticles (MNPs) with N,N-dimethylaminoethyl methacrylate (DMAEMA) and N-isopropylacrylamide (NIPAAm), best known for their pH- and thermo-sensitive properties, respectively. We also conjugated mesoporous silica nanoparticles (MSNs) to polymer matrix acting as drug container to enhance the drug encapsulation efficacy. Methotroxate (MTX) as a model drug was successfully loaded in MNCs (M-MNCs) via surface adsorption onto MSNs and electrostatic interaction between drug and carrier. The pH- and temperature-triggered release of MTX was concluded through the evaluation of in vitro release at both physiological and simulated tumor tissue conditions. Based on in vitro cytotoxicity assay results, M-MNCs significantly revealed higher antitumor activity compared to free MTX. In vitro MR susceptibility experiment showed that M-MNCs relatively possessed high transverse relaxivity (r2) of about 0.15 mM-1·ms-1 and a linear relationship between the transverse relaxation rate (R2) and the Fe concentration in the M-MNCs was also demonstrated. Therefore, the designed MNCs can potentially become smart drug carrier, while they also can be promising MRI negative contrast agent.
|
| |