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" A discrete cluster of urinary biomarkers discriminates between active systemic lupus erythematosus patients with and without glomerulonephritis. "
Landolt-Marticorena, Carolina; Prokopec, Stephenie D; Morrison, Stacey; Noamani, Babak; Bonilla, Dennisse; Reich, Heather; Scholey, James; Avila-Casado, Carmen; Fortin, Paul R; Boutros, Paul C; Wither, Joan
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AL
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| Record Number
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911520
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LA7704h34s
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| Title & Author
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A discrete cluster of urinary biomarkers discriminates between active systemic lupus erythematosus patients with and without glomerulonephritis. [Article]\ Landolt-Marticorena, Carolina; Prokopec, Stephenie D; Morrison, Stacey; Noamani, Babak; Bonilla, Dennisse; Reich, Heather; Scholey, James; Avila-Casado, Carmen; Fortin, Paul R; Boutros, Paul C; Wither, Joan
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2016
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| Title of Periodical
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UCLA
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| Abstract
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Management of lupus nephritis (LN) would be greatly aided by the discovery of biomarkers that accurately reflect changes in disease activity. Here, we used a proteomics approach to identify potential urinary biomarkers associated with LN.Urine was obtained from 60 LN patients with paired renal biopsies, 25 active non-LN SLE patients, and 24 healthy controls. Using Luminex, 128 analytes were quantified and normalized to urinary creatinine levels. Data were analyzed by linear modeling and non-parametric statistics, with corrections for multiple comparisons. A second cohort of 33 active LN, 16 active non-LN, and 30 remission LN SLE patients was used to validate the results.Forty-four analytes were identified that were significantly increased in active LN as compared to active non-LN. This included a number of unique proteins (e.g., TIMP-1, PAI-1, PF4, vWF, and IL-15) as well as known candidate LN biomarkers (e.g., adiponectin, sVCAM-1, and IL-6), that differed markedly (>4-fold) between active LN and non-LN, all of which were confirmed in the validation cohort and normalized in remission LN patients. These proteins demonstrated an enhanced ability to discriminate between active LN and non-LN patients over several previously reported biomarkers. Ten proteins were found to significantly correlate with the activity score on renal biopsy, eight of which strongly discriminated between active proliferative and non-proliferative/chronic renal lesions.A number of promising urinary biomarkers that correlate with the presence of active renal disease and/or renal biopsy changes were identified and appear to outperform many of the existing proposed biomarkers.
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